影像科学与光化学 ›› 2020, Vol. 38 ›› Issue (6): 941-949.DOI: 10.7517/issn.1674-0475.200514

• 综述与论文 • 上一篇    下一篇

金量子点的仿生化合成及在血清葡萄糖检测中的应用

朱韬1, 张艳2, 董露露1,3, 郭鹏越1, 杨冬芝1   

  1. 1. 徐州医科大学 新药研究与临床药学重点实验室, 江苏 徐州 221004;
    2. 中国人民解放军联勤保障部队904医院, 江苏 常州 213000;
    3. 常州第一人民医院, 江苏 常州 213000
  • 收稿日期:2020-05-21 出版日期:2020-11-15 发布日期:2020-11-15
  • 通讯作者: 杨冬芝
  • 基金资助:
    Xuzhou Natural Science Foundation (KC18201, KC18108,KC19066), and Jiangsu Undergraduate Innovation and Entrepreneurship Training Program (201910313012Z)

Biomimetic Synthesis of Au QDs and the Application of Glucose Detection in Serum

ZHU Tao1, ZHANG Yan2, DONG Lulu1,3, GUO Pengyue1, YANG Dongzhi1   

  1. 1. Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, Jiangsu, P. R. China;
    2. Hospital 904 of China Joint Logistic Support Force, Changzhou 213000, Jiangsu, P. R. China;
    3. The First People's Hospital of Changzhou, Changzhou 213000, Jiangsu, P. R. China
  • Received:2020-05-21 Online:2020-11-15 Published:2020-11-15
  • Supported by:
    Xuzhou Natural Science Foundation (KC18201, KC18108,KC19066), and Jiangsu Undergraduate Innovation and Entrepreneurship Training Program (201910313012Z)

摘要: 在纳米材料合成新方法研究中,仿生化合成因其明显优于化学合成的优点引起诸多研究者的关注。本文利用细胞生命代谢驱动在活细胞中制备金量子点(Au QDs)。结构表征显示,制备的金量子点具有立方晶体结构,尺寸分布均匀,直径约为20 nm。细胞实验表明,仿生化合成Au QDs的生物相容性明显高于传统化学方法。在一定条件下,葡萄糖对Au QDs的荧光具有猝灭作用,且荧光猝灭率与葡萄糖浓度呈良好的线性关系,线性范围为7.85×10-6~2.5×10-4 mol/L,检出限为3.1×10-8 mol/L。将本方法用于糖尿病大鼠血糖浓度检测,测定结果与血糖仪一致。本方法制备的Au QDs具有较低的细胞毒性和潜在检测能力,有望用于生物小分子的检测。

关键词: 仿生化合成, 金量子点, 肿瘤细胞, 葡萄糖

Abstract: In the research of new synthesis methods of non-toxic nanomaterials, biomimetic synthesis has attracted the attention of many researchers because of its obvious advantages over chemical synthesis. Herein gold quantum dots (Au QDs) were prepared in living cells driven by cell life metabolism. The characterization shows that the prepared gold Au QDs have cubic crystal structure, uniform size distribution and a diameter of about 20 nm. Cell experiments showed that the biocompatibility of Au QDs synthesized in cells was significantly higher than that of traditional chemical synthesis. Under certain conditions, the fluorescence of Au QDs can be quenched by glucose, and the fluorescence quenching efficiency of Au QDs shows a good linear relationship with glucose concentration, with a linear range of 7.85×10-6-2.5×10-4 mol/L and a detection limit of 3.1×10-8 mol/L. This method was applied to detect blood glucose concentration in diabetic rats, and the result was consistent with that of blood glucose meter. The prepared Au QDs have low cytotoxicity and potential detection ability, and can be used for the detection of small biological molecules.

Key words: biomimetic synthesis, gold quantum dots, cancer cell, glucose