氧化钼量子点无标记快速ATP检测

doi:10.7517/issn.1674-0475.200602

影像科学与光化学 ›› 2020, Vol. 38 ›› Issue (6): 950-955.DOI: 10.7517/issn.1674-0475.200602

氧化钼量子点无标记快速ATP检测

黄海清^1,2, 肖赛金^1,2, 杨光赵耀¹, 赵家辉¹

1. 东华理工大学核资源与环境省部共建国家重点实验室培育基地, 江西南昌 330013;
2. 东华理工大学, 化学生物与材料科学学院, 江西南昌 330013

收稿日期:2020-06-01 出版日期:2020-11-15 发布日期:2020-11-15
通讯作者: 肖赛金
基金资助:
江西省自然科学基金（20161BAB213090）和核资源与环境国家重点实验室开放基金（NRE1314）

Label-free and Rapid Detection of ATP Based on Molybdenum Oxide Quantum Dots

HUANG Haiqing^1,2, XIAO Saijin^1,2, YANG Guangzhaoyao¹, ZHAO Jiahui¹

1. State Key Laboratory Breeding Base of Nuclear Resources and Environment, East China University of Technology, Nanchang 330013, Jiangxi, P. R. China;
2. School of Chemistry, Biology and Material Science, East China University of Technology, Nanchang 330013, Jiangxi, P. R. China

Received:2020-06-01 Online:2020-11-15 Published:2020-11-15

摘要/Abstract

摘要： 以MoS₂和H₂O₂为原料，采用室温"一锅法"制备具有荧光性的氧化钼量子点（MoO_x QDs）。以MoO_x QDs为荧光探针，Cu²⁺为猝灭剂，构建了无标记"off-on"型ATP荧光检测。在pH为7.5，50 mol/L Cu²⁺，0.051 mg/mL MoO_x QDs的最优条件下，ATP浓度在25~250 mol/L时，520 nm处荧光强度会随ATP浓度增大而增强，且荧光强度与ATP浓度呈良好的线性关系，F=785.40+9.05c，R=0.993；BSA、Ni²⁺、Mn²⁺、Mg²⁺、Fe³⁺、Co²⁺、Ca²⁺等共存对ATP产生的干扰小。因此，"off-on"型MoO_x QDs-Cu²⁺检测平台可实现ATP的快速半定量检测，测试灵敏性高，稳定性好。

关键词: 三磷酸腺苷, 氧化钼量子点, 铜离子, 荧光探针

Abstract: Molybdenum oxide quantum dots (MoO_x QDs) with excellent fluorescence performance were synthesized using MoS₂ and H₂O₂ as the raw materials by a one-pot method at the room temperature. Based on the fluorescence "off-on" mode, a new rapid fluorescence detection of ATP was constructed, in which MoO_x QDs was a fluorescent probe and Cu²⁺ was a quencher. Under the optimized condition, pH 7.5, 0.051 mg/mL MoO_x QDs and 50 mol/L Cu²⁺, the fluorescence at 520 nm was gradually enhanced with increasing ATP concentration in the range of 25-250 mol/L. The calibration curve was F=785.40+9.05c with the correlation coefficient of 0.993. The coexistence of BSA, Ni²⁺, Mn²⁺, Mg²⁺, Fe³⁺, Co²⁺ and Ca²⁺ has negligible effect on ATP detection. Therefore, the "off-on" detection platform shows rapid and stable for sensing for ATP.

Key words: adenosine triphosphate, MoO_x quantum dots, Cu²⁺, fluorescent probe

黄海清, 肖赛金, 杨光赵耀, 赵家辉. 氧化钼量子点无标记快速ATP检测[J]. 影像科学与光化学, 2020, 38(6): 950-955.

HUANG Haiqing, XIAO Saijin, YANG Guangzhaoyao, ZHAO Jiahui. Label-free and Rapid Detection of ATP Based on Molybdenum Oxide Quantum Dots[J]. Imaging Science and Photochemistry, 2020, 38(6): 950-955.

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氧化钼量子点无标记快速ATP检测

Label-free and Rapid Detection of ATP Based on Molybdenum Oxide Quantum Dots

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