罗丹明6G螺环酰肼荧光快速检测谷氨酸和天门冬氨酸

doi:10.7517/j.issn.1674-0475.180103

影像科学与光化学 ›› 2018, Vol. 36 ›› Issue (4): 315-323.DOI: 10.7517/j.issn.1674-0475.180103

罗丹明6G螺环酰肼荧光快速检测谷氨酸和天门冬氨酸

谢先格¹, 夏方媛¹, 黄苏琴¹, 彭小伟¹, 傅燕¹, 王筱梅^1,2, 陶绪堂²

1. 苏州科技大学化学生物与材料工程学院, 江苏苏州 215009;
2. 山东大学晶体材料国家重点实验室, 山东济南 250100

收稿日期:2018-01-09 修回日期:2018-03-25 出版日期:2018-07-15 发布日期:2018-07-15
通讯作者: 王筱梅

Rapid Fluorescence Detection of Glutamic Acid and Aspartic Acid by Spirocyclic Rhodamine 6G Hydrazide

XIE Xiange¹, XIA Fangyuan¹, HUANG Suqin¹, PENG Xiaowei¹, FU Yan¹, WANG Xiaomei^1,2, TAO Xutang²

1. Institute of Chemistry, Biology and Materials Engineering, Suzhou University of Science and Technology, Suzhou 215009, Jiangsu, P. R. China;
2. State Key Laboratory of Crystal Materials, Shandong University, Ji'nan 250100, Shandong, P. R. China

Received:2018-01-09 Revised:2018-03-25 Online:2018-07-15 Published:2018-07-15

摘要/Abstract

摘要： 以罗丹明6G螺环酰肼为荧光探针，在水溶液中可选择性检测谷氨酸和天门冬氨酸。在500 nm波长激发下，1 min内两种样品溶液即发出550 nm绿光，检测限分别可达7×10^-8 mol/L和1.4×10^-7 mol/L。此外本文还分析了罗丹明6G螺环酰肼能够选择性和高灵敏度快速荧光检测谷氨酸和天门冬氨酸的机理。

关键词: 罗丹明6G螺环酰肼, 荧光探针, 谷氨酸, 天门冬氨酸, 快速荧光检测

Abstract: A spirocyclic rhodamine 6G hydrazide was used as fluorescence probe to detect glutamic acid and aspartic acid in aqueous solution. Both sample solution emitted 550 nm green light in 1 minute (the excitation wavelength is 500 nm).The detection limit can be as low as 7×10^-8 mol/L and 1.4×10^-7 mol/L respectively. Finally, the mechanism of selective and rapid fluorescence detection for glutamic acid and aspartic acid was analyzed.

Key words: spirocyclic rhodamine 6G hydrazide, fluorescence probe, glutamic acid, aspartic acid, rapid fluorescence detection

谢先格, 夏方媛, 黄苏琴, 彭小伟, 傅燕, 王筱梅, 陶绪堂. 罗丹明6G螺环酰肼荧光快速检测谷氨酸和天门冬氨酸[J]. 影像科学与光化学, 2018, 36(4): 315-323.

XIE Xiange, XIA Fangyuan, HUANG Suqin, PENG Xiaowei, FU Yan, WANG Xiaomei, TAO Xutang. Rapid Fluorescence Detection of Glutamic Acid and Aspartic Acid by Spirocyclic Rhodamine 6G Hydrazide[J]. Imaging Science and Photochemistry, 2018, 36(4): 315-323.

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罗丹明6G螺环酰肼荧光快速检测谷氨酸和天门冬氨酸

Rapid Fluorescence Detection of Glutamic Acid and Aspartic Acid by Spirocyclic Rhodamine 6G Hydrazide

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