镝荧光探针的构建及对牛奶中四环素残留的检测

doi:10.7517/j.issn.1674-0475.2014.06.565

影像科学与光化学 ›› 2014, Vol. 32 ›› Issue (6): 565-571.DOI: 10.7517/j.issn.1674-0475.2014.06.565

• 应用与发展 • 上一篇

镝荧光探针的构建及对牛奶中四环素残留的检测

王曼丽¹, 李鑫¹, 张肖会¹, 明华蜜¹, 尹洪宗¹, 徐坤²

1. 山东农业大学化学与材料科学学院, 山东泰安 271000;
2. 山东农业大学园艺科学与工程学院, 山东泰安 271000

收稿日期:2014-04-25 修回日期:2014-06-17 出版日期:2014-11-15 发布日期:2014-11-15
通讯作者: 尹洪宗, 徐坤
基金资助:
国家自然科学基金(31171953)项目资助

Construction of Dysprosium Fluorescent Probe and Application of Determination on Tetracrycline Hydrochlorid Residues in Milk

WANG Manli¹, LI Xin¹, ZHANG Xiaohui¹, MING Huami¹, YIN Hongzong¹, XU Kun²

1. College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, Shandong, P.R.China;
2. College of Horticulture Science and Engineering, Shandong Agricultural University, Taian 271018, Shandong, P.R.China

Received:2014-04-25 Revised:2014-06-17 Online:2014-11-15 Published:2014-11-15

摘要/Abstract

摘要：

在乙醇体系中构建三元配合物的镝荧光探针,并利用盐酸四环素(TC)对镝荧光探针具有荧光猝灭作用,提出了一种检测牛奶中四环素残留的新方法.首先确定体系的激发波长为305 nm,发射波长为574 nm.在配比、加料顺序、时间等方面对镝荧光探针进行了条件优化,确定了镝离子(Dy³⁺)、磺基水杨酸(SSA)、三正辛基氧化磷(TOPO)的最佳配比为1:2:0.1和30 min最佳检测时间.其次建立了检测盐酸四环素的线性曲线,并获得检测范围为10^-6~2×10^-5mol/L.最后,利用此荧光探针对处理的牛奶样品进行加样回收检测,回收率在96.9 %~104.4 %.实验证明建立的方法科学可行,对盐酸四环素具有高选择性.

关键词: 镝, 荧光探针, 盐酸四环素(TC), 牛奶

Abstract:

A novel method for the detection of tetracycline was described on the basis of ternary dysprosium fluorescent probe. The tetracycline hydrochloride has flurescence quenching effect for dysprosium fluorescent probe in ethonal system. The excitation wavelength of the system was at 305 nm and the emission wavelength at 574 nm, respectively. The optimized condition were measured: the best ration of dysprosium (Dy³⁺), sulfosalicylic acid (SSA), Tri-n-octyl phosphine oxide (TOPO) was 1:2:0.1, and the best fluorescence detection time was 30 min. Moreover, the calibration curve of detection for tetracycline hydrochloride was established which ranged from 1×10^-6 to 2×10^-5 mol/L. The dysprosium fluorescent probe was utilized to conduct the sample recovery test of the treated milk samples, the recovery of the milk sample test was 96.9 %104.4 %. As a result, the experimental research demonstrated that the method constructed is scientific and feasible, and has high selectivity to the tetracycline hydrochloride.

Key words: dysprosium, fluorescent probe, hydrochloric acid tetracycline, milk

王曼丽, 李鑫, 张肖会, 明华蜜, 尹洪宗, 徐坤. 镝荧光探针的构建及对牛奶中四环素残留的检测[J]. 影像科学与光化学, 2014, 32(6): 565-571.

WANG Manli, LI Xin, ZHANG Xiaohui, MING Huami, YIN Hongzong, XU Kun. Construction of Dysprosium Fluorescent Probe and Application of Determination on Tetracrycline Hydrochlorid Residues in Milk[J]. Imaging Science and Photochemistry, 2014, 32(6): 565-571.

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镝荧光探针的构建及对牛奶中四环素残留的检测

Construction of Dysprosium Fluorescent Probe and Application of Determination on Tetracrycline Hydrochlorid Residues in Milk

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