肿瘤细胞内CdS纳米量子点的仿生化合成

doi:10.7517/j.issn.1674-0475.2018.01.005

影像科学与光化学 ›› 2018, Vol. 36 ›› Issue (1): 57-63.DOI: 10.7517/j.issn.1674-0475.2018.01.005

肿瘤细胞内CdS纳米量子点的仿生化合成

纪海侠^1,2, 董露露^1,2, 杜岩^1,2, 姚欣悦^1,2, 杨冬芝^1,2

1. 徐州医科大学药物分析教研室, 江苏徐州 221004;
2. 徐州医科大学新药研究与临床药学重点实验室, 江苏徐州 221004

收稿日期:2017-02-21 修回日期:2017-05-02 出版日期:2018-01-15 发布日期:2018-01-15
通讯作者: 杨冬芝
基金资助:
国家自然科学基金青年基金项目（81201696）、江苏省自然科学基金项目（BK20161173）和江苏省"六大人才高峰"高层次人才项目（2014-SWYY-007）资助

Biomimetic Synthesis of CdS Quantum Dots in Living Cancer Cells

JI Haixia^1,2, DONG Lulu^1,2, DU Yan^1,2, YAO Xinyue^1,2, YANG Dongzhi^1,2

1. Department of Pharmaceutical Analysis, Xuzhou Medical University, Xuzhou 221004, Jiangsu, P. R. China;
2. Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, Jiangsu, P. R. China

Received:2017-02-21 Revised:2017-05-02 Online:2018-01-15 Published:2018-01-15

摘要/Abstract

摘要： 以宫颈癌HeLa细胞为载体，细胞内源性谷胱甘肽为保护剂和稳定剂，在细胞生命代谢活动驱动下合成性质优良的CdS量子点。光学性能表征显示，该CdS量子点在波长为450 nm左右有发射峰，其荧光产率为5.2%。透射电镜表征显示CdS量子点粒径约为3.0 nm，X射线衍射光谱表明该CdS量子点为立方晶型结构。

关键词: 仿生合成, 硫化镉量子点, 肿瘤细胞, 谷胱甘肽

Abstract: With the cervical cancer cells, HeLa, as the carrier, glutathione in living cancer cells as the modifier and stabilizer, CdS quantum dots was synthesized during the normal cells life metabolic process. The optical spectra showed that the prepared CdS quantum dots emitted at about 450 nm with the quantum yield of 5.2%. The structure characteristics by transmission electron microscopy (TEM) and X-ray powder diffraction (XRD) showed that the particles size of the prepared CdS quantum dots was about 3.0 nm with cubic crystal structure.

Key words: biomimetic synthesis, cadmium sulfide, cancer cells, glutathione

纪海侠, 董露露, 杜岩, 姚欣悦, 杨冬芝. 肿瘤细胞内CdS纳米量子点的仿生化合成[J]. 影像科学与光化学, 2018, 36(1): 57-63.

JI Haixia, DONG Lulu, DU Yan, YAO Xinyue, YANG Dongzhi. Biomimetic Synthesis of CdS Quantum Dots in Living Cancer Cells[J]. Imaging Science and Photochemistry, 2018, 36(1): 57-63.

参考文献

[1] Nam K T, Kim D W, Yoo P J, Chiang C Y, Meethong N, Hammond P T, Chiang Y M, Belcher A M. Virus-enabled synthesis and assembly of nanowires for lithium ion battery electrodes[J]. Science, 2006, 312(5775):885-888.
[2] Sweeney RY, Mao C, Gao X, Burt J L, Belcher A M, Georgiou G, Iverson B L. Bacterial biosynthesis of cadmium sulfide nanocrystals[J]. Chemistry & Biology, 2004, 11(11):1553-1559.
[3] Williams P, Keshavarz-Moore E, Dunnill P. Efficient production of microbially synthesized cadmium sulfide quantum semiconductor crystallites[J]. Enzyme And Microbial Technology, 1996, 19(3):208-213.
[4] Tan L, Wan A, Li H. Synthesis of near-infrared quantum dots in cultured cancer cells[J]. ACS Applied Materials & Interfaces, 2014, 6(1):18-23.
[5] Anshup A, Venkataraman J S, Subramaniam C, Kumar R R, Priya S, Kumar T R, Omkumar R V, John A, Pradeep T. Growth of gold nanoparticles in human cells[J]. Langmuir, 2005, 21(25):11562-11567.
[6] Li Y,Cui R, Zhang P, Chen B B, Tian Z Q, Li L, Hu B, Pang D W, Xie Z X. Mechanism-oriented controllability of intracellular quantum dots formation:the role of glutathione metabolic pathway[J]. ACS Nano, 2013, 7(3):2240-2248.
[7] Perez-Donoso J M, Monras J P, Bravo D, Aguirre A, Quest A F, Osorio-Román I O, Aroca R F, Chasteen T G, Vásquez C C.Biomimetic, mild chemical synthesis of CdTe-GSH quantum dots with improved biocompatibility[J]. Plos One, 2012, 7(1):e30741.
[8] Monras J P, Diaz V, Bravo D,Montes R A, Chasteen T G, Osorio-Román I O, Vásquez C C, Pérez-Donoso J M.Enhanced glutathione content allows the in vivo synthesis of fluorescent CdTe nanoparticles by escherichia coli[J]. Plos One, 2012, 7(11):e48657.
[9] Sturzenbaum S R, Hockner M, Pannerselvam A, Levitt J, Bouillard J S, Taniguchi S, Dailey L A, Ahmad Khanbeigi R, Rosca E V, Thanou M, Suhling K, Zayats A V, Green M. Biosynthesis of luminescent quantum dots in an earthworm[J]. Nature Nanotechnology, 2013, 8(1):57-60.
[10] Qian J, Cao Y, Chen Z, Liu C, Lu X. Biomimetic synthesis of cerium oxide nanosquares on RGO and their enhanced photocatalytic activities[J]. Dalton Transactions, 2017, 46(2):547-553.
[11] He X, Ma N. Biomimetic synthesis of fluorogenic quantum dots for ultrasensitive label-free detection of protease activities[J]. Small, 2013, 9(15):2527-2531.
[12] Stroh M, Zimmer J P, Dudad G,Levchenko T S, Cohen K S, Brown E B, Scadden D T, Torchilin V P, Bawendi M G, Fukumura D, Jain R K.Quantum dots spectrally distinguish multiple species within the tumor milieu in vivo[J]. Nature Medicine, 2005, 11(6):678-682.
[13] Murray C B, Norris D J, Bawendi M G. Synthesis and characterization of nearly monodisperse CdE (E=sulfur, selenium, tellurium) semiconductor nanocrystallites[J]. Journal of the American Chemical Society, 1993, 115(19):8706-8715.
[14] Peng Z A, Peng X G. Formation of high-quality CdTe, CdSe, and CdS nanocrystals using CdO as precursor[J]. Journal of the American Chemical Society, 2001, 123(1):183-184.
[15] Xianyu Y, Xie Y, Wang N, Wang Z, Jiang X. A dispersion-dominated chromogenic strategy for colorimetric sensing of glutathione at the nanomolar level using gold nanoparticles[J]. Small, 2015, 11(41):5510-5514.
[16] GuoY, Wang H, Sun Y S,Qu B. A disulfide bound-molecular beacon as a fluorescent probe for the detection of reduced glutathione and its application in cells[J]. Chemical Communications, 2012, 48:3221-3223.
[17] Liu Z, Zhou X, Miao Y, Hu Y, Kwon N, Wu X, Yoon J. A reversible fluorescent probe for real-time quantitative monitoring of cellular glutathione[J]. Angewandte Chemie-International Edition, 2017,doi:10.1002/anie.201702114.
[18] DeGroot M W, Taylor N J, Corrigan J F.Molecular nanocluster analogues of CdSe/ZnSe and CdTe/ZnTe core/shellnanoparticles[J]. Journal of Materials Chemistry, 2004, 14:654-660.
[19] Liu F C, Cheng T L, Shen C C,Tseng W L, Chiang M Y.Synthesis of cysteine-capped Zn_xCd_1-xSe alloyed quantum dots emitting in the blue-green spectral range[J]. Langmuir, 2008, 24(5):2162-2167.
[20] Deng Z T, Lie F L, Shen S Y, Ghosh I, Mansuripur M, Muscat A J.Water-based route to ligand-selective synthesis of ZnSe and Cd-doped ZnSe quantum dots with tunable ultraviolet A to blue photoluminescence[J]. Langmuir, 2009, 25(1):434-442.
[21] Demas J N, Crosby G A. The measurement of photoluminescence quantum yields[J]. Journal of Physical Chemistry,1971, 75(8):991-1024.
[22] Rosenthal S J. Bar-coding biomolecules with fluorescent nanocrystals[J]. Nature Biotechnology, 2001, 19(7):621-622.

肿瘤细胞内CdS纳米量子点的仿生化合成

Biomimetic Synthesis of CdS Quantum Dots in Living Cancer Cells

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