影像科学与光化学 ›› 2016, Vol. 34 ›› Issue (4): 319-328.DOI: 10.7517/j.issn.1674-0475.2016.04.319

• 论文 • 上一篇    下一篇

应用于生物发酵在线监测的聚合物pH传感膜

叶文1,2, 王书文1, 潘梦旭1, 赵平1, 彭阳峰2, 朱为宏1   

  1. 1. 华东理工大学 精细化工研究所, 上海 200237;
    2. 华东理工大学 化学工程学院, 上海 200237
  • 收稿日期:2016-04-03 修回日期:2016-04-29 出版日期:2016-07-15 发布日期:2016-07-15
  • 通讯作者: 赵平
  • 基金资助:

    国家自然科学青年基金项目(61306022)资助

Polymeric pH Sensor Film for Real-time Monitoring in Biological Culture

YE Wen1,2, WANG Shuwen1, PAN Mengxu1, ZHAO Ping1, PENG Yangfeng2, ZHU Weihong1   

  1. 1. Institute of Fine Chemicals, East China University of Science & Technology, Shanghai 200237, P.R.China;
    2. School of Chemical Engineering, East China University of Science & Technology, Shanghai 200237, P.R.China
  • Received:2016-04-03 Revised:2016-04-29 Online:2016-07-15 Published:2016-07-15

摘要:

微型生物反应器的成功操作依赖于对培养液pH值的监测和控制,聚合物pH荧光探针可以制成传感膜安装在微型反应器上,具有高通量筛选、非入侵性、容易集成等特征。甲基丙烯酸羟乙酯和N-(2-甲基丙烯酰乙酯基)-4-(N-甲基哌嗪基)-1,8-萘酰亚胺(NI)共聚形成的聚合物,具有良好亲水性、成膜性和生物相容性,并在pH=6~8之间具有良好的响应性能和稳定性。为了进一步提高膜的响应速率,将含有金刚烷的单体引入聚合物中,结果显示随着侧链金刚烷基团的含量增加,共聚物膜的pH响应时间变长;而将强亲水性的聚乙二醇引入共聚合物中,发现共聚物膜的pH响应速率得到了提高。将此pH传感膜应用于酵母发酵液中进行测试,具有良好的pH值在线监测性能。

关键词: 微型生物反应器, 聚合物, 荧光探针, pH传感膜, 响应速率

Abstract:

Successful operation of microbioreactors is critically dependent on the monitoring and control of pH of the culture fluids. Polymeric pH fluorescent probe can be easily fabricated into films and fixed onto microbioreactors. Copolymer P(HEMA-NI) has good hydrophilicity, film-forming property and biological compatibility, along with good response and repeatability in the pH range of 6-8 in the biological fermentation tank. In order to improve the response rate of pH polymer film, the third monomer was incorporated into the polymer matrix.The results showed that the pH response rate of films decreased with the incorporation of adamantine, while with the incorporation of hydrophilic PEG into the polymer, the response rate was significantly improved. These pH sensor films showed good pH on-line monitoring performance in yeast culture.

Key words: microbioreactor, polymer, fluorescent, pH sensing, response rate