影像科学与光化学 ›› 2018, Vol. 36 ›› Issue (5): 453-463.DOI: 10.7517/issn.1674-0475.180403

• 论文 • 上一篇    

TCMePyP的制备及对神经母细胞瘤细胞系SH-SY5Y光动力杀伤作用的研究

王琪1, 肖雁2, 肖勃1, 肖竦1   

  1. 1. 贵州医科大学 基础医学院 化学教研室, 贵州 贵阳 550025;
    2. 贵州医科大学 分子生物学重点实验室, 贵州 贵阳 550001
  • 收稿日期:2018-04-07 修回日期:2018-04-28 出版日期:2018-09-15 发布日期:2018-09-15
  • 通讯作者: 肖竦
  • 基金资助:
    国家自然科学基金(81660207)和贵州省科技厅联合基金项目([2015]7352)资助

Preparation of TCMePyP and Research on the Killing Effect of Photodynamic Therapy on SH-SY5Y Neuroblastoma Cell

WANG Qi1, XIAO Yan2, XIAO Bo1, XIAO Song1   

  1. 1. Department of Chemistry, School of Basic Medical Science, Guizhou Medical University, Guiyang 550025, Guizhou, P. R. China;
    2. The key Laboratory of Medical Molecular Biology, Guiyang 550001, Guizhou, P. R. China
  • Received:2018-04-07 Revised:2018-04-28 Online:2018-09-15 Published:2018-09-15

摘要: 在H2TPyP上接入羧基进行修饰,得到TCMePyP。测定了TCMePyP的紫外-可见吸收光谱、荧光光谱。观察了SH-SY5Y细胞对新型卟啉化合物的摄取情况和光照条件下细胞内活性氧物质(Reactive oxygen species,ROS)的变化情况。进一步考察了新型卟啉化合物对神经母细胞瘤细胞系SH-SY5Y的抑制作用。结果表明:TCMePyP具备良好光敏药物的特性,脂溶性和水溶性良好;在可见光区有较强吸收;光动力条件下活性氧物质ROS随光照时间在一定范围内增加;TCMePyP对SH-SY5Y肿瘤细胞的增殖有抑制作用,杀伤效果明显;发射的荧光在SH-SY5Y肿瘤细胞内可以定位成像。因此,TCMePyP具有较好的医药应用前景。

关键词: 卟啉化合物, 神经母细胞瘤, 光动力杀伤作用, 细胞内成像, 活性氧物质

Abstract: Carboxyl was connected to H2TPyP to modify and obtained the TCMePyP. UV-Vis absorption spectra and fluorescence spectra of TCMePyP were determined. The uptake of new porphyrin compounds in SH-SY5Y cells and the changes of intracellular ROS(reactive oxygen species) under radiation were observed. The inhibitory effect of new porphyrin compounds on neuroblastoma cell line SH-SY5Y was further investigated. The results showed that TCMePyP has the characteristics of effective photosensitizer:good lipid solubility and water solubility, strong absorption in visible light area. ROS increased with the radiation time under the photodynamic condition. It has inhibitory effect on the proliferation of SH-SY5Y tumor cells, and the killing effect is obvious. The emitted fluorescence can be located in SH-SY5Y tumor cells. In a word, TCMePyP has good prospects for medical applications.

Key words: porphyrin compound, neuroblastoma cell, killing effect of photodynamic therapy, cell imaging, ROS (reactive oxygen species)