荧光标记聚苯乙烯微球的激光捕获及其原位稳态和时间分辨荧光光谱研究

doi:10.7517/j.issn.1674-0475.2010.06.416

影像科学与光化学 ›› 2010, Vol. 28 ›› Issue (6): 416-424.DOI: 10.7517/j.issn.1674-0475.2010.06.416

荧光标记聚苯乙烯微球的激光捕获及其原位稳态和时间分辨荧光光谱研究

李明^1,2, 曾毅¹, 李迎迎¹, 李嫕¹

1. 中国科学院, 理化技术研究所, 光化学转换与光功能材料重点实验室, 北京, 100190;
2. 中国科学院, 研究生院, 北京, 100049

收稿日期:2010-05-12 修回日期:2010-05-28 出版日期:2010-11-23 发布日期:2010-11-23
通讯作者: 李嫕;李迎迎
基金资助:
中国科学院重大科研装备研制项目;中国科学院知识创新工程青年人才领域前沿项目(TYF0805);国家重点基础研究发展规划项目(2010CB934502)

Laser Trapping of Dye-Doped Polystyrene Beads and in Situ Measurements of Steady-State and Time-Resolved Fluorescence Spectroscopies

LI Ming^1,2, ZENG Yi¹, LI Ying-ying¹, LI Yi¹

1. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R.China;
2. Graduate University of the Chinese Academy of Sciences, Beijing 100049, P.R.China

Received:2010-05-12 Revised:2010-05-28 Online:2010-11-23 Published:2010-11-23

摘要/Abstract

摘要： 本工作设计研制了一套激光捕获与原位稳态及时间分辨荧光光谱检测系统,该系统将激光捕获技术与稳态及时间分辨荧光光谱检测技术相结合,实现了对亚微米级微粒的原位稳态及时间分辨发光性质研究.利用研制的仪器研究了荧光标记聚苯乙烯微球的激光捕获过程、以及捕获微粒的原位稳态及时间分辨荧光光谱.本工作为单个纳米粒子发光性质的研究开辟了一条新的途径.

关键词: 激光捕获, 稳态荧光光谱, 时间分辨荧光光谱, 荧光标记聚苯乙烯微球, 罗丹明6G

Abstract: An experimental setup of laser trapping combined with in situ measurements of steady-state and time-resolved fluorescence spectroscopies was developed for analyzing photophysical properties of a single submicrometer particle in solution.Simultaneous laser trapping and in situ steady-state and time-resolved fluorescence measurements of dye-doped polystyrene beads were demonstrated.This study provides a new strategy for investigating photophysical properties of a single submicrometer particle.

Key words: laser trapping, steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, dye-doped polystyrene bead,rhodamine 6G

中图分类号:

李明, 曾毅, 李迎迎, 李嫕. 荧光标记聚苯乙烯微球的激光捕获及其原位稳态和时间分辨荧光光谱研究[J]. 影像科学与光化学, 2010, 28(6): 416-424.

LI Ming, ZENG Yi, LI Ying-ying, LI Yi. Laser Trapping of Dye-Doped Polystyrene Beads and in Situ Measurements of Steady-State and Time-Resolved Fluorescence Spectroscopies[J]. Imaging Science and Photochemistry, 2010, 28(6): 416-424.

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荧光标记聚苯乙烯微球的激光捕获及其原位稳态和时间分辨荧光光谱研究

Laser Trapping of Dye-Doped Polystyrene Beads and in Situ Measurements of Steady-State and Time-Resolved Fluorescence Spectroscopies

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