影像科学与光化学 ›› 2014, Vol. 32 ›› Issue (1): 106-112.DOI: 10.7517/j.issn.1674-0475.2014.01.106

• 论文 • 上一篇    下一篇

基于花菁的硫醇近红外比率荧光探针

朱东建1, 江华1,2   

  1. 1. 北京分子科学国家实验室, 中国科学院 化学研究所 光化学重点实验室, 北京 100190;
    2. 北京师范大学 化学学院, 北京 100875
  • 收稿日期:2013-12-05 修回日期:2013-12-17 出版日期:2014-01-15 发布日期:2014-01-15
  • 通讯作者: 江华
  • 基金资助:

    国家自然科学基金项目(21102148,21125205)和科技部973研究项目(2011CB935800)资助

Cyanine-based Near-infrared Ratiometric Fluorescent Probe for Thiols

ZHU Dongjian1, JIANG Hua1,2   

  1. 1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
    2. College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China
  • Received:2013-12-05 Revised:2013-12-17 Online:2014-01-15 Published:2014-01-15

摘要:

本文设计合成了硫醇的近红外比率荧光探针CySS。CySS以七甲川花菁为荧光发色团,二硫键为硫醇的反应位点。通过对其性质和应用的详细深入研究,结果表明,探针分子CySS具有灵敏度高,选择性好,且不受pH影响等优点,并且能成功地应用到活细胞内硫醇的检测。

关键词: 硫醇, 花菁, 近红外, 比率, 荧光探针

Abstract:

The article describes the design and synthesis of a near-infrared ratiometric fluorescent probe CySS for thiols, the probe CySS has heptamethine cyanine as the reporter, a disulfide group as the thiol reaction site. The properties and application of CySS were studied in detail. The results demonstrate that CySS displays high sensitivity, good selectivity, and pH-independent. Moreover, we have demonstrated that CySS is suitable for imaging thiols in living cells, providing a potentially powerful tool for thiols detection in biological systems.

Key words: thiols, cyanine, near-infrared, ratiometric, fluorescent probe