pH值响应的共轭聚合物纳米粒子的制备、表征与细胞成像研究

doi:10.7517/j.issn.1674-0475.2014.01.121

影像科学与光化学 ›› 2014, Vol. 32 ›› Issue (1): 121-130.DOI: 10.7517/j.issn.1674-0475.2014.01.121

• 论文 • 上一篇

pH值响应的共轭聚合物纳米粒子的制备、表征与细胞成像研究

王建武, 朱春雷, 冯丽恒, 袁焕祥, 吕凤婷, 刘礼兵, 王树

北京分子科学国家实验室, 中国科学院化学研究所有机固体重点实验室, 北京 100190

收稿日期:2013-12-16 修回日期:2013-12-23 出版日期:2014-01-15 发布日期:2014-01-15
通讯作者: 王树

Preparation, Characterization and Cell Imaging of pHresponsive Conjugated Polymer Nanoparticles

WANG Jianwu, ZHU Chunlei, FENG Liheng, YUAN Huanxiang, LU Fengting, LIU Libing, WANG Shu

Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China

Received:2013-12-16 Revised:2013-12-23 Online:2014-01-15 Published:2014-01-15

摘要/Abstract

摘要：

共轭聚合物纳米粒子（CPNs）因其高荧光亮度、低毒性、表面易修饰的特性，近年来在生物材料和生物医药领域备受关注。本论文中我们设计、合成了一种新的pH 值响应共轭聚合物（PFPA），并通过纳米沉淀方法制备了其纳米粒子。动态光散射实验表明PFPA纳米粒子在水中分散性较好，其粒径约为8 nm。 PFPA纳米粒子的最大吸收峰为379 nm，其摩尔吸光系数为2.1×10⁶ L·mol-1·cm-1;另外该纳米粒子的荧光最大发射峰为422 nm，其荧光量子产率为35%。PFPA纳米粒子在汞灯（100瓦）照射下表现出较好的光稳定性，另外MTT实验表明其具有较低的细胞毒性。该纳米粒子具有pH响应的光学特性，并可以用于活细胞成像。PFPA纳米粒子在癌症诊断、药物与基因传递等方面具有潜在的应用价值。

关键词: 共轭聚合物纳米粒子, 荧光探针, pH响应, 细胞成像

Abstract:

Conjugated polymer nanoparticles (CPNs) have attracted much attention in biomaterials and biomedical fields owning to their high brightness, low cytotoxicity and easy modification properties. Herein, a new conjugated polymer PFPA was synthesized and the nanoparticles were prepared using a nanoprecipitation method. Dynamic light scattering experiment indicates that PFPA nanoparticles possess small size with an average diameter of 8 nm in water. PFPA nanoparticles exhibit maximum absorption peak at 379 nm with a molar absorption coefficient of 2.1×10⁶ L·mol^-1·cm^-1 and maximum emission peak at 422 nm with a fluorescence quantum yield of 35%. Moreover, PFPA nanoparticles exhibit a satisfactory photostability under the irradiation of mercury lamp (100 W), and also display less cytotoxicity. In the aspect of applications, pHresponsive optical behavior and cell imaging ability make PFPA nanoparticles an attractive candidate for cancer diagnosis, drug and gene delivery.

Key words: conjugated polymer nanoparticles, fluorescence probe, pHresponsive, cell imaging

王建武, 朱春雷, 冯丽恒, 袁焕祥, 吕凤婷, 刘礼兵, 王树. pH值响应的共轭聚合物纳米粒子的制备、表征与细胞成像研究[J]. 影像科学与光化学, 2014, 32(1): 121-130.

WANG Jianwu, ZHU Chunlei, FENG Liheng, YUAN Huanxiang, LU Fengting, LIU Libing, WANG Shu. Preparation, Characterization and Cell Imaging of pHresponsive Conjugated Polymer Nanoparticles[J]. Imaging Science and Photochemistry, 2014, 32(1): 121-130.

参考文献

[1] Stender A S, Marchuk K, Liu C, et al. Single cell optical imaging and spectroscopy[J]. Chemical Reviews, 2013, 113(4): 2469-2527.
[2] Fan L J, Zhang Y, Murphy C B, et al. Fluorescent conjugated polymer molecular wire chemosensors for transition metal ion recognition and signaling[J]. Coordination Chemistry Reviews, 2009, 253(3): 410-422.
[3] An L, Liu L, Wang S. Cationic conjugated polymers for homogeneous and sensitive fluorescence detection of hyaluronidase[J]. Science in China Series B: Chemistry, 2009, 52: 827-832.
[4] Feng X, Liu L, Wang S, et al. Water-soluble fluorescent conjugated polymers and their interactions with biomacromolecules for sensitive biosensors[J]. Chemical Society Reviews, 2010, 39(7): 2411-2419.
[5] Zhu C, Yang Q, Lü F, et al. Conjugated polymer-coated bacteria for multimodal intracellular and extracellular anticancer activity[J]. Advanced Materials, 2013, 25(8): 1203-1208.
[6] Zhu C, Liu L, Yang Q, et al. Water-soluble conjugated polymers for imaging, diagnosis, and therapy[J]. Chemical Reviews, 2012, 112(8): 4687-4735.
[7] Wen Q, Tang H, Yang G, et al. Synthesis and characterization of oligofluorene nanoparticles for cell imaging[J]. Acta Chimica Sinica, 2012, 70: 2137-2143.
[8] Yu M, Tang Y, He F, et al. Synthesis of water-soluble dendritic conjugated polymers for fluorescent DNA assays[J]. Macromolecular Rapid Communications, 2006, 27(20): 1739-1745.
[9] Zhu B, Han Y, Sun M, et al. Water-soluble dendronized polyfluorenes with an extremely high quantum yield in water[J]. Macromolecules, 2007, 40(13): 4494-4500.[ZK)]
[10] [ZK(#]Tuncel D, Demir H V. Conjugated polymer nanoparticles[J]. Nanoscale, 2010, 2(4): 484-494.
[11] Feng L, Zhu C, Yuan H, et al. Conjugated polymer nanoparticles: preparation, properties, functionalization and biological applications[J]. Chemical Society Reviews, 2013, 42(16): 6620-6633.
[12] Xing C, Liu L, Tang H, et al. Design guidelines for conjugated polymers with light-activated anticancer activity[J]. Advanced Functional Materials, 2011, 21(21): 4058-4067.
[13] Chong H, Nie C, Zhu C, et al. Conjugated polymer nanoparticles for light-activated anticancer and antibacterial activity with imaging capability[J]. Langmuir, 2012, 28(4): 2091-2098.
[14] Wu C, Bull B, Szymanski C, et al. Multicolor conjugated polymer dots for biological fluorescence imaging[J]. ACS Nano, 2008, 2(11): 2415-2423.
[15] Lee Y J, Kang H C, Hu J, et al. pH-sensitive polymeric micelle-based pH probe for detecting and imaging acidic biological environments[J]. Biomacromolecules, 2012, 13(9): 2945-2951.
[16] Lee E S, Oh K T, Kim D, et al. Tumor pH-responsive flower-like micelles of poly(L-lactic acid)-b-poly(ethylene glycol)-b-poly(L-histidine)[J]. Journal of Controlled Release, 2007, 123(1): 19-26.
[17] Wen Q, Liu L, Yang Q, et al. Dopamine-modified cationic conjugated polymer as a new platform for pH sensing and autophagy imaging[J]. Advanced Functional Materials, 2013, 23(6): 764-769.
[18] Lu H, Xu B, Dong Y, et al. Novel fluorescent pH sensors and a biological probe based on anthracene derivatives with aggregation-induced emission characteristics[J]. Langmuir, 2010, 26(9): 6838-6844.
[19] Panchuk-Voloshina N, Haugland R P, Bishop-Stewart J, et al. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates[J]. Journal of Histochemistry & Cytochemistry, 1999, 47(9): 1179-1188.
[20] Du J Z, Sun T M, Song WJ, et al. A tumor-acidity-activated charge-conversional nanogel as an intelligent vehicle for promoted tumoral-cell uptake and drug delivery[J]. Angewandte Chemie-International Edition, 2010, 49(21): 3621-3626.
[21] Mintzer M A, Simanek E E. Nonviral vectors for gene delivery[J]. Chemical Reviews, 2008, 109(2): 259-302.
[22] Pu K Y, Liu B. A multicolor cationic conjugated polymer for naked-eye detection and quantification of heparin[J]. Macromolecules, 2008, 41(18): 6636-6640.

pH值响应的共轭聚合物纳米粒子的制备、表征与细胞成像研究

Preparation, Characterization and Cell Imaging of pHresponsive Conjugated Polymer Nanoparticles

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	黄海清, 肖赛金, 杨光赵耀, 赵家辉. 氧化钼量子点无标记快速ATP检测[J]. 影像科学与光化学, 2020, 38(6): 950-955.
[2]	王凯霞, 徐炜, 贺庆国, 程建功. 高选择性甲基苯丙胺蒸气检测用荧光探针的设计、合成及性能[J]. 影像科学与光化学, 2018, 36(6): 478-488.
[3]	常顺周, 相开强, 明伟, 张志忠, 田宝柱, 张金龙. 一种基于PET机理pH荧光探针的合成及其性能研究[J]. 影像科学与光化学, 2018, 36(5): 425-433.
[4]	谢先格, 夏方媛, 黄苏琴, 彭小伟, 傅燕, 王筱梅, 陶绪堂. 罗丹明6G螺环酰肼荧光快速检测谷氨酸和天门冬氨酸[J]. 影像科学与光化学, 2018, 36(4): 315-323.
[5]	马亚光, 张玉涛, 马一宇, 郭志前, 朱为宏. 基于菁染料高选择性检测半胱氨酸的近红外荧光探针[J]. 影像科学与光化学, 2018, 36(3): 236-244.
[6]	崔丽霞, 张红红, 李苗, 张彩红, 张国梅, 双少敏, 董川. pH比率荧光探针的制备及光谱研究[J]. 影像科学与光化学, 2018, 36(3): 283-290.
[7]	卢香, 郝春玲, 姜小明. 氨基酸型表面活性剂烷基二羧酸钠的结构对表面活性、自聚集行为及润湿性能的影响[J]. 影像科学与光化学, 2018, 36(3): 291-297.
[8]	冯佳, 蔚芳, 王煜. 一种增强型荧光探针对Al³⁺的识别检测及细胞成像研究[J]. 影像科学与光化学, 2018, 36(2): 178-186.
[9]	邱申保, 王素敏, 王奇观, 尚嘉茵, 张文治, 杨磊, 樊亚茹. 水溶性氮掺杂石墨烯量子点的合成及其对Pb²⁺的选择性识别研究[J]. 影像科学与光化学, 2018, 36(1): 108-116.
[10]	黄振涛, 王楠楠, 张健, 赵伟利. 用于检测三苯基膦的一种新型“点亮”型荧光探针[J]. 影像科学与光化学, 2017, 35(6): 771-779.
[11]	崔倩玲, 叶荣琴, 李立东. 基于能量转移机理的水溶性荧光共轭聚合物体系的设计制备及应用[J]. 影像科学与光化学, 2017, 35(4): 429-444.
[12]	张健, 吕明环, 张维娟, 赵伟利. 基于黄酮的ESIPT型荧光探针检测三苯基膦[J]. 影像科学与光化学, 2017, 35(4): 526-535.
[13]	王媛, 陈潇潇, 刘学良, 陈玉哲, 牛丽亚, 吴骊珠, 杨清正. 选择性检测谷胱甘肽的荧光探针[J]. 影像科学与光化学, 2017, 35(4): 536-545.
[14]	费强, 安建才, 赵春常. 基于新型不对称BODIPY的GSH荧光探针[J]. 影像科学与光化学, 2017, 35(4): 546-551.
[15]	何业, 唐俊马, 郭志前, 靳鹏伟, 朱世琴, 朱为宏. 基于罗丹明和BODIPY单元的FRET探针的合成及其对小分子α-酮戊二酸的检测[J]. 影像科学与光化学, 2017, 35(3): 265-273.