影像科学与光化学 ›› 2010, Vol. 28 ›› Issue (1): 1-9.DOI: 10.7517/j.issn.1674-0475.2010.01.1

• 研究快讯 •    下一篇

竹红菌素金属配合物与人血清白蛋白相互作用的光谱研究

金咪咪, 欧植泽, 高云燕, 郝萍, 殷卫峰, 刘丽华   

  1. 西北工业大学, 理学院, 应用化学系, 陕西, 西安, 710072
  • 收稿日期:2009-11-12 修回日期:2009-12-08 出版日期:2010-01-23 发布日期:2010-01-23
  • 通讯作者: OU Zhi-ze, Tel & Fax:(029)88431677.

Spectroscopic Study on the Interaction of Metal Complexes of Hypocrellin A with Human Serum Albumin

JIN Mi-mi, OU Zhi-ze, GAO Yun-yan, HAO Ping, YIN Wei-feng, LIU Li-hua   

  1. Department of Applied Chemistry, School of Science, Northwestern Polytechnical University, Xi’an 710072, Shanxi, P.R.China
  • Received:2009-11-12 Revised:2009-12-08 Online:2010-01-23 Published:2010-01-23

摘要: 利用多种光谱方法研究了Mg2+-HA,Y3+-HA,La3+-HA等竹红菌甲素金属离子配合物(M-HA)与人血清白蛋白(HSA)之间的相互作用.结果表明在pH 7.4和5.0条件下M-HA能够与HSA形成1:1的复合物,其结合常数均大于2×104L/mol.电子自旋共振和荧光猝灭实验表明HSA和M-HA在激发态条件下具有光诱导电子转移作用.

关键词: 竹红菌素, 人血清白蛋白, 静电相互作用, 光诱导电子转移, 荧光猝灭

Abstract: Interaction of metal complexes of hypocrellin A(M-HA),such as Mg2+-HA, Y3+-HA,La3+-HA with human serum albumin(HSA) is studied by spectroscopic methods. The results suggest that M-HA and HSA can form 1:1 complex with moderate association constants(>2×104 L/mol) at pH 7.4 and 5.0.Electron paramagnetic resonance (EPR) and fluorescence quenching experiments indicate that photoinduced electron transfer from HSA to M-HA may occur at excited state.

Key words: hypocrellin, human serum albumin, electrostatic interaction, photoinduced electron transfer, fluorescence quenching

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