基于罗丹明B的激活型α-酮戊二酸荧光探针

doi:10.7517/j.issn.1674-0475.2015.03.195

摘要/Abstract

摘要：

α-酮戊二酸 (α-KA)是人体三羧酸循环的一个重要代谢中间体,异柠檬酸脱氢酶-1 (IDH1)及异柠檬酸脱氢酶-2 (IDH2)的突变将导致α-KA转化为2-羟基戊二酸 (2HG),该过程与急性骨髓性白血病 (AML)密切相关,因此检测人体内α-KA的含量变化具有重要意义.本文以罗丹明B作为荧光基团,通过将罗丹明B与水合肼反应得到能够检测α-KA的荧光增强型探针RBN,并优化了检测温度、pH及响应时间等条件.研究表明,RBN对α-KA表现出很好的选择性,不受人体内常见氨基酸、化学结构类似的羰基化合物及活性氧化物 (ROS)的影响,具有潜在的应用价值.

关键词: 罗丹明B, &alpha, -酮戊二酸, 荧光探针, 激活型

Abstract:

As a dicarboxyl metabolite produced by living cells, α-ketoglutaratc acid (α-KA) can be converted to 2-hydroxyglutarate (2HG) which is confirmed to be associated with the acute myeloid leukemia (AML). In this paper, a rhodamine B-based turn-on fluroescent sensor for α-KA has been designed and synthesized, which is consist of rhodamine-B and hydrazino group. After a series of parallel optimization such as response time, pH and temperature, RBN shows an obvious turn-on fluorescence response to α-KA. In addition, the sensor exhibits good selectivity towards α-KA among 20 common amino acids, various dicarbonyl derivatives and reactive oxygen species.

Key words: rhodamine B, α-ketoglutaric acid, fluroescence probe, turn-on

焦长红, 何业, 靳鹏伟, 朱世琴, 朱为宏. 基于罗丹明B的激活型α-酮戊二酸荧光探针[J]. 影像科学与光化学, 2015, 33(3): 195-202.

JIAO Changhong, HE Ye, JIN Pengwei, ZHU Shiqin, ZHU Weihong. A Turn-on Fluorescent Probe for α-Ketoglutaric Acid Based on Rhodamine B[J]. Imaging Science and Photochemistry, 2015, 33(3): 195-202.

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