心衰标志物可视化联合检测方法研究

doi:10.7517/j.issn.1674-0475.2015.04.275

影像科学与光化学 ›› 2015, Vol. 33 ›› Issue (4): 275-284.DOI: 10.7517/j.issn.1674-0475.2015.04.275

心衰标志物可视化联合检测方法研究

霍卫松^1,2, 张磊^1,2, 高宇哲^1,2, 孟凡达^1,2, 高云华¹

1. 中国科学院理化技术研究所光化学转换和功能材料重点实验室, 北京 100190;
2. 中国科学院大学, 北京 100049

收稿日期:2015-05-21 修回日期:2015-05-28 出版日期:2015-07-16 发布日期:2015-07-16
通讯作者: 高云华
基金资助:
国家科技支撑计划(2013BAI03B)资助

Rapid Visual Immunoassay of Multi-biomarker for Heart Failure Based on Magnetic Particles Images

HUO Weisong^1,2, ZHANG Lei^1,2, GAO Yuzhe^1,2, MENG Fanda^1,2, GAO Yunhua¹

1. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
2. University of Chinese Academy of Sciences, Beijing 100049, P. R. China

Received:2015-05-21 Revised:2015-05-28 Online:2015-07-16 Published:2015-07-16

摘要/Abstract

摘要：

基于夹心免疫原理和表面免疫吸附模型,采用Image Pro Plus软件对硅片表面磁颗粒分布图像进行定量化处理,建立了硅片表面心衰标志物可视化联合检测方法。研究表明,该方法从抗体包被到获得检测结果不超过1 ｈ,操作简单,在单张硅片上可实现心衰标志物NT-proBNP和cTnI的联合检测。NT-proBNP和cTnI的最低检出限分别为21.8 pg/mL和0.034 ng/mL,检测范围分别为21.8~40000 pg/mL和0.034~50 ng/mL。83例不同程度心血管疾病患者的血清样本的检测结果与商品化免疫分析仪检测结果一致,相关系数r>0.98。因此,该快速免疫检测方法可以满足临床检测心衰标志物的需要。

关键词: 硅生物芯片, 可视化, 免疫检测方法, 心衰, 生物标志物

Abstract:

Based on sandwich immunoassay, immuno-adsorption model and quantitative analysis of magnetic particles image on the surface of silicon chip, rapid immunoassay of multi-biomarkers for heart failure had been established.This method is simple and rapid compared to traditional ELISA.The assay time does not exceed 1 hour from antibody immobilization to final results obtained. In addition, it was easy to realize a joint measurement for NT-proBNP and cTnI on single silicon biochip.The limits of detection for NT-proBNP and cTnI were 21.8 pg/mL and 0.034 ng/mL, and the detection ranges were 21.840000 pg/mL and 0.03450 ng/mL, respectively.The assay results for NT-proBNP and cTnIwere compared using 83 serum samples from patients with different cardiovascular diseases with those on commercial analyzers and obtained a very good correlation (r>0.98). In conclusion, this rapid visual immunoassay is well suitable for the clinical detection of heart failure biomarkers.

Key words: silicon biochip, visualization, immunoassay, heart failure, biomarker

霍卫松, 张磊, 高宇哲, 孟凡达, 高云华. 心衰标志物可视化联合检测方法研究[J]. 影像科学与光化学, 2015, 33(4): 275-284.

HUO Weisong, ZHANG Lei, GAO Yuzhe, MENG Fanda, GAO Yunhua. Rapid Visual Immunoassay of Multi-biomarker for Heart Failure Based on Magnetic Particles Images[J]. Imaging Science and Photochemistry, 2015, 33(4): 275-284.

[1]	高文宇, 周奕华, 滕潇, 吴丽辉, 曹晟. 基于纸基的荧光碳量子点传感器的可视化检测研究进展[J]. 影像科学与光化学, 2018, 36(4): 367-379.
[2]	李丹丹, 陈鑫, 王宏, 付杨, 余愿, 只金芳. 纳米金刚石拉曼生物探针用于抗菌肽杀菌过程的可视化研究[J]. 影像科学与光化学, 2018, 36(1): 89-99.
[3]	俞丹萍, 刘亚伟, 申亮, 李富友. 花菁染料负载的聚合物纳米材料用于次氯酸可视化检测的研究[J]. 影像科学与光化学, 2017, 35(3): 290-296.

心衰标志物可视化联合检测方法研究

Rapid Visual Immunoassay of Multi-biomarker for Heart Failure Based on Magnetic Particles Images

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价