纳米ZnS与明胶蛋白质的相互作用

doi:10.7517/j.issn.1674-0475.2016.04.336

摘要/Abstract

摘要：

利用透射电子显微镜(TEM)、X射线粉末衍射(XRD)、紫外-可见吸收光谱(UV-Vis)、傅里叶变换红外光谱(FT-IR)、同步荧光和三维荧光光谱,研究了pH=7.40条件下纳米ZnS与明胶蛋白质的相互作用。FT-IR光谱表明,ZnS与明胶中酰胺基的氮和氧原子及羧基氧可能发生键合生成了水溶性ZnS/明胶纳米生物复合物;ZnS与明胶的键合对明胶分子的内源荧光有明显的猝灭作用,其猝灭机制为静态猝灭。根据修正的Scatchard方程计算了不同温度下复合物的生成常数及反应热力学参数,结果表明反应是自发进行的吸热过程,且为熵驱动。同步荧光和三维荧光光谱表明纳米ZnS引起了明胶蛋白质构象的改变。研究结果为探究此类纳米生物复合物的制备及其在生物方面的应用提供了重要信息。

关键词: ZnS, 明胶, ZnS/明胶纳米生物复合物, 荧光光谱

Abstract:

The interaction between ZnS nanoparticles and gelatin was investigated at pH=7.40 by TEM, XRD, UV-Vis, FT-IR, synchronous fluorescence and three-dimensional fluorescence spectroscopy. FT-IR spectra provide the evidence that ZnS probably has the direct chemical bonding reaction with the nitrogen and oxygen atoms, and carboxyl oxygen in gelatin macromolecule, to form ZnS/gelatin bionanocomposites. The intrinsic fluorescence of gelatin was obviously quenched by ZnS nanoparticles, which is a static quenching procedure. The apparent thermodynamic formation constants and thermodynamic parameters at different temperatures were calculated by the modified Scatchard equation, and the results indicated that the reaction was spontaneous and entropy-driven. The synchronous fluorescence and three-dimensional fluorescence spectra showed that the gelatin conformation changed after binding with ZnS. The results can provide important information for exploring the preparation and biological application of this kind of bionanocomposites.

Key words: ZnS, gelatin, ZnS/gelatin bionanocomposite, fluorescence spectroscopy

邓秋, 黎幼群, 唐世华. 纳米ZnS与明胶蛋白质的相互作用[J]. 影像科学与光化学, 2016, 34(4): 336-345.

DENG Qiu, LI Youqun, TANG Shihua. Interaction Between ZnS Nanoparticles and Gelatin[J]. Imaging Science and Photochemistry, 2016, 34(4): 336-345.

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