激光共聚焦显微拉曼快速测定食用调和油脂肪酸

doi:10.7517/j.issn.1674-0475.2017.02.147

影像科学与光化学 ›› 2017, Vol. 35 ›› Issue (2): 147-152.DOI: 10.7517/j.issn.1674-0475.2017.02.147

激光共聚焦显微拉曼快速测定食用调和油脂肪酸

董晶晶, 吴静珠, 陈岩, 刘翠玲

北京工商大学食品安全大数据技术北京市重点实验室, 北京 100048

收稿日期:2017-01-10 修回日期:2017-02-15 出版日期:2017-03-15 发布日期:2017-03-15
通讯作者: 吴静珠
基金资助:
北京市自然科学基金面上项目（4132008）、北京教委重点项目（KZ201310011012）和北京工商大学研究生科研能力提升计划项目（2016）

Rapid Determination of Edible Oil Fatty Acids Using Confocal Laser Microscopy Raman Spectroscopy

DONG Jingjing, WU Jingzhu, CHEN Yan, LIU Cuiling

Beijing Key Laboratory of Big Data Technology for Food Safety, School of Computer and Information Engineering, Beijing Technology and Business University, Beijing 100048, P. R. China

Received:2017-01-10 Revised:2017-02-15 Online:2017-03-15 Published:2017-03-15

摘要/Abstract

摘要：

目的：采用激光共聚焦显微拉曼光谱技术快速测定食用调和油饱和脂肪酸（Saturated fatty acids，SFA）、单不饱和脂肪酸（Monounsaturated fatty acids，MUFA）和多不饱和脂肪酸（Polyunsaturated fatty acids，PUFA）含量及比例。方法：通过导数预处理净化拉曼光谱信息，采用偏最小二乘法建立优化后的SFA、MUFA、PUFA的拉曼定量预测模型，为计算脂肪酸比例提供准确的数据基础。结果：SFA、MUFA和PUFA定量分析模型的决定系数R²均大于0.99，相对分析误差RPD均大于3，表明模型具有较高的稳定性和良好的预测能力。结论：激光拉曼光谱法结合化学计量学方法可以快速、准确地测定食用调和油SFA、MUFA、PUFA含量及比例，为快速检测食用调和油品质提供切实可行的检测手段。

关键词: 食用调和油, 拉曼光谱, 偏最小二乘法, 饱和脂肪酸, 不饱和脂肪酸

Abstract:

Objective:To study using the confocal laser microscopy Raman Spectroscopy for Rapid Analysis of edible oil saturated fatty acids(SFA), monounsaturated fatty acids(MUFA)and polyunsaturated fatty acid(PUFA) the content and proportion. Methods:Cleaning Raman spectroscopy information by derivative pretreatment, using partial least squares to establish optimized Raman quantitative prediction model of SFA, MUFA, PUFA, which providing accurate data base for the calculation of the proportion of fatty acids. Results:Determination coefficient R² of SFA, MUFA and PUFA quantitative analysis model were greater than 0.99, the RPD is greater than 3, indicating that the model has high stability and good predictive ability. Conclusion:Laser Raman spectroscopy combined with chemometrics methods can quickly and accurately determine the content and proportion of edible oil SFA, MUFA, PUFA, providing a practical detection methods for the rapid detection of edible oil quality.

Key words: edible oil, Raman spectroscopy, partial least squares, saturated fatty acids, unsaturated fatty acids

董晶晶, 吴静珠, 陈岩, 刘翠玲. 激光共聚焦显微拉曼快速测定食用调和油脂肪酸[J]. 影像科学与光化学, 2017, 35(2): 147-152.

DONG Jingjing, WU Jingzhu, CHEN Yan, LIU Cuiling. Rapid Determination of Edible Oil Fatty Acids Using Confocal Laser Microscopy Raman Spectroscopy[J]. Imaging Science and Photochemistry, 2017, 35(2): 147-152.

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激光共聚焦显微拉曼快速测定食用调和油脂肪酸

Rapid Determination of Edible Oil Fatty Acids Using Confocal Laser Microscopy Raman Spectroscopy

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