影像科学与光化学 ›› 2017, Vol. 35 ›› Issue (6): 843-852.DOI: 10.7517/j.issn.1674-0475.2017.05.008

• 论文 • 上一篇    下一篇

基于金属纳米粒子增敏的SPR生物传感器检测吲哚乙酸

高文慧, 王兆群, 泮俊青, 尹洪宗   

  1. 山东农业大学 化学与材料科学学院, 山东 泰安 271018
  • 收稿日期:2016-12-28 修回日期:2017-04-10 出版日期:2017-11-15 发布日期:2017-11-15
  • 通讯作者: 尹洪宗
  • 基金资助:

    国家自然科学基金项目(31171953)资助

Determination of Indole Acetic Acid by SPR Biosensor Based on the Metal Nanoparticles Sensitization

GAO Wenhui, WANG Zhaoqun, PAN Junqing, YIN Hongzong   

  1. College of Chemistry and Material Science, Shandong Agricultural University, Taian 201718, Shandong, P. R. China
  • Received:2016-12-28 Revised:2017-04-10 Online:2017-11-15 Published:2017-11-15

摘要:

本实验建立了表面等离子体共振(SPR)生物传感器检测3-吲哚乙酸(IAA)的方法。制备了两种SPR生物传感器检测IAA:传统模式的SPR生物传感器1和Au/Ag合金纳米粒子增敏的SPR生物传感器2。结果发现:传感器1在IAA浓度范围为175~350 μg/L时,浓度与其波数位移值呈线性关系,检出限为25 μg/L(S/N=3);传感器2在IAA浓度范围为17.5~250 μg/L时,浓度与其波数位移值呈线性关系,检出限为2.2 μg/L(S/N=3)。说明基于Au/Ag合金纳米粒子的传感器2比传感器1有较高的灵敏度和较低的检出限。加标回收实验测得加标回收率范围为96%~100.2%,平均值为98.4%。本实验制备的SPR生物传感器具有较好的精密度、稳定性、重现性和特异性。

关键词: 表面等离子体共振生物传感器, 吲哚乙酸, 吲哚乙酸抗体, Au/Ag合金纳米粒子

Abstract:

Surface plasmon resonance (SPR) biosensor was a new method for detecting 3-indole acetic acid (IAA). We adopted two kinds of indole acetic acid SPR biosensors to detect IAA:Traditional sensor 1 and Au/Ag alloy nanoparticles based sensor 2. Sensor 1:The wavenumber shifts were linearly proportional to the IAA concentration in a range from 175 to 350 μg/L with a detection limit of 25 μg/L (S/N=3). Sensor 2:The wavenumber shifts were linearly proportional to the IAA concentration in a range from 17.5 to 250 μg/L with a detection limit of 2.2 μg/L (S/N=3). The results showed that the sensor 2 based on Au/Ag alloy nanoparticles had higher sensitivity and lower detection limit than the the sensor 1. The rate of standard addition recovery was 96%-100.2%, the average was 98.4%. The prepared SPR biosensor had good precision, stability, repeatability and specificity.

Key words: SPR biosensor, indole acetic acid (IAA), IAA antibody, Au/Ag alloy nanoparticles