影像科学与光化学 ›› 2018, Vol. 36 ›› Issue (1): 89-99.DOI: 10.7517/j.issn.1674-0475.2018.01.009

• 论文 • 上一篇    下一篇

纳米金刚石拉曼生物探针用于抗菌肽杀菌过程的可视化研究

李丹丹1,2, 陈鑫1,2,3, 王宏3, 付杨1,2, 余愿1, 只金芳1   

  1. 1. 中国科学院 理化技术研究所, 北京 100190;
    2. 中国科学院大学, 北京 100049;
    3. 北京大学 药学院, 北京 100191
  • 收稿日期:2017-02-21 修回日期:2017-04-27 出版日期:2018-01-15 发布日期:2018-01-15
  • 通讯作者: 只金芳
  • 基金资助:
    国家自然科学基金项目(21375137)和东南大学生物电子国家重点实验室开放基金资助

Nanodiamonds Raman Probes for Visualizing the Interaction between Antimicrobial Peptide and Bacteria

LI Dandan1,2, CHEN Xin1,2,3, WANG Hong3, FU Yang1,2, YU Yuan1, ZHI Jinfang1   

  1. 1. Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
    2. University of Chinese Academy of Sciences, Beijing 100049, P. R. China;
    3. School of Pharmaceutical Sciences, Peking University, Beijing 100191, P. R. China
  • Received:2017-02-21 Revised:2017-04-27 Online:2018-01-15 Published:2018-01-15

摘要: 本工作以纳米金刚石为探针,依赖拉曼成像技术,成功实现了对细菌体系生命活动的观察。实验中将抗菌肽死亡素负载于100 nm的金刚石上,利用纳米金刚石在1332 cm-1处的特征拉曼信号为标记,通过共聚焦拉曼成像技术可视化了纳米金刚石-死亡素复合物与枯草芽孢杆菌间的相互作用过程。同时,采用扫描电子显微镜观察手段验证了上述拉曼成像方法的有效性。此外,抗菌实验验证了纳米金刚石-死亡素复合物对枯草芽孢杆菌有达到45%的明显杀灭效果,表明纳米金刚石探针的引入不会影响死亡素的抗菌性能。本工作证实了纳米金刚石拉曼生物探针用于观察抗菌过程的可行性,为其在生物成像领域中的应用提供了重要依据。

关键词: 纳米金刚石, 拉曼生物探针, 抗菌肽, 杀菌活性, 可视化

Abstract: This article realizes the application of nanodiamonds (NDs) Raman probes for vital activity observation in bacteria system. The antimicrobial peptide thanatin was immobilized on 100 nm NDs, and the interaction of the NDs-thanatin conjugate with Bacillus subtilis was visualized via Raman imaging with the intrinsic diamond Raman signal (1332 cm-1) as label. Scanning electron microscope was further utilized to verify the practicability of Raman mapping. Besides, antibacterial activity of NDs-thanatin conjugate was validated, suggesting the adsorbed thanatin on NDs remained superior capability. The results showed the feasibility of nanodiamonds as Raman probes to visualize the bacteria activity, indicating the potential use of NDs in bio-imaging applications.

Key words: nanodiamonds, Raman probes, antimicrobial peptide, antibacterial activity, visualization