一种增强型荧光探针对Al3+的识别检测及细胞成像研究

doi:10.7517/j.issn.1674-0475.2018.02.007

摘要/Abstract

摘要： 以2-羟基-1-萘甲醛和2-氨甲基吡啶为原料合成了一种简单的希夫碱化合物（L）。L作为增强型荧光探针，在缓冲水溶液中可选择性检测Al³⁺，检测限为80.5 nmol/L，远低于世界卫生组织规定的饮用水中Al³⁺的上限值（7.41 μmol/L）。通过荧光共聚焦成像，化合物L可高效地检测人体SiHa癌细胞内的Al³⁺。

关键词: 希夫碱, 荧光探针, 识别检测, Al³⁺, 细胞成像

Abstract: Based on the inhibition of C=N isomerization and chelation-enhanced fluorescence (CHEF), the simple Schiff base 1-(2-pyridylmethyleneimino) naphthalen-2-ol (L) acts as an effective fluorometric sensor for Al³⁺ in buffered semi-aqueous medium. L exhibited selective fluorescence enhancement upon binding Al³⁺as a detection response, which giving strong blue emission (438 nm). The sensitivity of the fluorescent based assay (80.5 nmol/L) for Al³⁺ is far below the limit recommended in the World Health Organization (WHO) guidelines for drinking water (7.41 μmol/L). L was successfully applied to detect intracellular Al³⁺ in human SiHa cells through fluorescence microscopic imaging. The interaction between the sensor and Al³⁺ has been investigated in detail by NMR and HRMS analysis.

Key words: Schiff base, fluorescence probe, detection, Al³⁺, cell imaging

冯佳, 蔚芳, 王煜. 一种增强型荧光探针对Al³⁺的识别检测及细胞成像研究[J]. 影像科学与光化学, 2018, 36(2): 178-186.

FENG Jia, YU Fang, WANG Yu. A Turn-on Fluorometric Sensor for Al³⁺ in Aqueous Medium with Cell Imaging Application[J]. Imaging Science and Photochemistry, 2018, 36(2): 178-186.

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一种增强型荧光探针对Al³⁺的识别检测及细胞成像研究

A Turn-on Fluorometric Sensor for Al³⁺ in Aqueous Medium with Cell Imaging Application

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