影像科学与光化学 ›› 2018, Vol. 36 ›› Issue (3): 283-290.DOI: 10.7517/j.issn.1674-0475.2018.03.009

• 论文 • 上一篇    下一篇

pH比率荧光探针的制备及光谱研究

崔丽霞1, 张红红1, 李苗1, 张彩红1, 张国梅1, 双少敏1, 董川2   

  1. 1. 山西大学 化学化工学院, 山西 太原 030006;
    2. 山西大学 环境科学研究所, 山西 太原 030006
  • 收稿日期:2017-11-14 修回日期:2018-01-09 出版日期:2018-05-15 发布日期:2018-05-15
  • 通讯作者: 张彩红, 董川
  • 基金资助:
    山西省回国留学人员科研资助项目(2017-011)和山西省自然科学基金(201601D011017)项目资助

Preparation and Spectral Characterization of a pH Ratiometric Fluorescence Probe

CUI Lixia1, ZHANG Honghong1, LI Miao1, ZHANG Caihong1, ZHANG Guomei1, SHUANG Shaomin1, DONG Chuan2   

  1. 1. School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, Shanxi, P. R. China;
    2. Institute of Environmental Science, Shanxi University, Taiyuan 030006, Shanxi, P. R. China
  • Received:2017-11-14 Revised:2018-01-09 Online:2018-05-15 Published:2018-05-15

摘要: 以吲哚(苯并吲哚)衍生物和4-羟基苯甲醛为原料,通过一步反应合成半花菁类pH荧光探针12。两个探针水溶性好、发光性能稳定,它们的紫外吸收光谱和荧光光谱都能比率检测酸性和碱性溶液pH变化,探针1的两个pKa为3.81和10.01,探针2的两个pKa为4.03和10.47。两个pH探针都具有Stokes位移大、灵敏度高、可逆性好等特点。二者相比,探针1对碱性更敏感,探针2对酸性更敏感。

关键词: 比率荧光探针, 半花菁, pH变化, 紫外光谱, 荧光光谱

Abstract: Fluorescent probes (1 and 2) for pH based on semi-cyanine were synthesized from indole (benzo indole) derivatives and 4-hydroxybenzaldehyde using one-step condensation reaction. The probes have good water solubility and stable luminescence properties. They can be used to detect pH of acidic and alkaline solutions by the UV absorption spectra or ratiometric fluorescence spectra. The pKa data of probe 1 are 3.81 and 10.01, and the pKa data of probe 2 are 4.03 and 10.47. Both pH probes have some advantages, such as large Stokes shift, high sensitivity and good reversibility. Compared the two probes, probe 1 is more sensitive to the alkalinity, and probe 2 is more sensitive to the acidity.

Key words: ratio fluorescence probes, semi-cyanine, pH changes, UV spectra, fluorescence spectra