碳点-核酸/氧化石墨烯荧光探针用于miRNA-21的细胞成像

doi:10.7517/j.issn.1674-0475.180104

影像科学与光化学 ›› 2018, Vol. 36 ›› Issue (4): 350-358.DOI: 10.7517/j.issn.1674-0475.180104

碳点-核酸/氧化石墨烯荧光探针用于miRNA-21的细胞成像

胡先运^1,2,3, 孟铁宏¹, 王传明¹, 张汝国¹, 李炜⁴, 吕廷勇⁴, 江家志¹, 莫兴泽¹, 龙友国¹, 余跃生¹, 潘卫东^2,3

1. 黔南民族医学高等专科学校, 贵州都匀 558000;
2. 贵州省中国科学院天然产物化学重点实验室, 贵州贵阳 550002;
3. 贵州医科大学天然产物化学重点实验室, 贵州贵阳 550002;
4. 黔南民族医学高等专科学校附属医院, 贵州都匀 558000

收稿日期:2018-01-11 修回日期:2018-04-23 出版日期:2018-07-15 发布日期:2018-07-15
通讯作者: 胡先运
基金资助:
国家自然科学基金项目（21545006）、贵州省自然科学基金（黔科合基础[2016]1136号，黔科合J字[2015]2138号）、黔南州优青项目、黔南科技计划项目（黔南科合工字[2016]12号，黔南科合社字[2016]1号）和黔南民族医专科研基金（QNYZ201601，QNYZ201605，QNYZ201801，QNYZ201807）资助

CD-DNA/GO Fluorescent Nanoprobe for Detection of miRNA-21 in situ Live Cell

HU Xianyun^1,2,3, MENG Tiehong¹, WANG Chuanming¹, ZHANG Ruguo¹, LI Wei⁴, LÜ Tingyong⁴, JIANG Jiazhi¹, MO Xingze¹, LONG Youguo¹, YU Yuesheng¹, PAN Weidong^2,3

1. Qiannan Medical College for Nationalities, Duyun 558000, Guizhou, P. R. China;
2. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550002, Guizhou, P. R. China;
3. The Key Laboratory of Chemistry for Natural Products of Guizhou Medical university, Guiyang 550002, Guizhou, P. R. China;
4. Affiliated Hospital of Qiannan Medical College for Nationality, Duyun 558000, Guizhou, P. R. China

Received:2018-01-11 Revised:2018-04-23 Online:2018-07-15 Published:2018-07-15

摘要/Abstract

摘要： 通过生物偶联方式，将无毒的碳点（Carbon Dots，CD）表面功能化上核酸，以核酸功能化的碳点（CD-DNA）为荧光团，氧化石墨烯（GO）为猝灭剂，二者组装成CD-DNA/GO纳米荧光探针，CD与GO之间产生长程共振能量转移（long-range resonance energy transfer，LrRET），CD荧光猝灭。当miRNA-21存在时，DNA与其杂交，CD从GO表面分离，二者之间的荧光共振能量转移被打断，碳点荧光恢复，通过荧光"开"方式检测miRNA-21，检测限为0.2 nmol/L，线性范围为1～500 nmol/L。该探针成功应用于血癌K562细胞中miRNA-21的原位成像，为血癌的原位诊断和治疗提供了研究依据。

关键词: 碳点, 氧化石墨烯, 荧光成像, DNA, miRNA检测

Abstract: By using carbon dots (CD) to label DNA as fluorophore by biological coupling method, combining with graphene oxide (GO) as quencher, CD-DNA/GO fluorescent nanoprobe was constructed, and developed for miRNA-21 detection. In CD-DNA/GO, the fluorescence of CD was quenched by GO through long-range resonance energy transfer (LrRET). In present of miRNA-21, the DNA bind with miRNA-21 to form duplex, releasing CD-DNA from GO and interrupting the LrRET, and the fluorescence of CD was recovery to achieve miRNA-21 detection by fluorescence "turn on" mode. The obtained the linear range for miRNA-21 was from 1 nmol/L to 500 nmol/L; the detection of limit was estimated to be 0.2 nmol/L by the miRNA-21 fluorescent nanoprobe. The nanoprobe was achieved for detection of miRNA-21 in situ live cell imaging, which provide the basic research for in situ diagnosis and treatment of leukemia.

Key words: carbon dots, graphene oxide, fluorescent imaging, DNA, miRNA detection

胡先运, 孟铁宏, 王传明, 张汝国, 李炜, 吕廷勇, 江家志, 莫兴泽, 龙友国, 余跃生, 潘卫东. 碳点-核酸/氧化石墨烯荧光探针用于miRNA-21的细胞成像[J]. 影像科学与光化学, 2018, 36(4): 350-358.

HU Xianyun, MENG Tiehong, WANG Chuanming, ZHANG Ruguo, LI Wei, LÜ Tingyong, JIANG Jiazhi, MO Xingze, LONG Youguo, YU Yuesheng, PAN Weidong. CD-DNA/GO Fluorescent Nanoprobe for Detection of miRNA-21 in situ Live Cell[J]. Imaging Science and Photochemistry, 2018, 36(4): 350-358.

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碳点-核酸/氧化石墨烯荧光探针用于miRNA-21的细胞成像

CD-DNA/GO Fluorescent Nanoprobe for Detection of miRNA-21 in situ Live Cell

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