石墨烯/壳聚糖电化学传感器对色氨酸对映体的选择性识别

doi:10.7517/issn.1674-0475.180402

摘要/Abstract

摘要： 本文设计合成了一种石墨烯/壳聚糖复合物薄膜，并将其作为电化学电极应用于选择性检测具有旋光活性的色氨酸（Trp）对映体。采用X射线衍射、红外光谱、拉曼光谱、X射线光电子能谱、透射和扫描电子显微镜表征了石墨烯/壳聚糖复合物的结构。以石墨烯/壳聚糖复合物为电极，通过示差脉冲伏安法实现了Trp对映异构体的识别，结果表明L-Trp比D-Trp具有更高的电化学响应，并且峰电流密度和Trp的浓度在0.3×10^-4~1.5×10^-4 mol·L^-1范围内有线性关系。该复合物电极具有厚度和面积可控、稳定性高等优点，可显著提高电化学检测中的电流信号，有望用于分子药物及临床医学领域中手性对映体的识别。

关键词: 石墨烯, 壳聚糖, 示差脉冲伏安法, 氨基酸, 选择性识别

Abstract: In this research, graphene/chitosan composite films were designed and used as electrode for electrochemical enantioselective recognition of tryptophan (Trp) enantiomers. The structure of the graphene/chitosan composite films were characterized using X-ray diffraction, FT-IR spectroscopy, Raman spectroscopy, XPS spectroscopy, transmission and scanning electron microscopy. Trp enantiomers were recognized using graphene/chitosan composite electrode by a differential pulse voltammetry. Experimental data indicated that L-Trp exhibited stronger electrochemical response than D-Trp, and that a linear relationship between the current density of the oxidative peak and the concentrations of L-/D-Trp was established in a concentration span ranging from 0.3×10^-4 mol·L^-1 to 1.5×10^-4 mol·L^-1. The composite electrode had a number of advantages including controllable thickness and area, good stability, and enhanced electrochemical signals. Therefore, the graphene/chitosan composite electrodes are expected to be used for enantioselective recognition in areas such as molecular drugs and clinical medicine.

Key words: graphene, chitosan, differential pulse voltammetry, amino acids, enantiomeric recognition

张金萍, 范景彪, 黄艳, 米红宇, 耿建新. 石墨烯/壳聚糖电化学传感器对色氨酸对映体的选择性识别[J]. 影像科学与光化学, 2018, 36(5): 398-407.

ZHANG Jinping, FAN Jingbiao, HUANG Yan, MI Hongyu, GENG Jianxin. Electrochemical Selective Recognition of Tryptophan Enantiomers Based on Graphene/Chitosan Composite Films[J]. Imaging Science and Photochemistry, 2018, 36(5): 398-407.

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