激光共聚焦显微技术在共定位应用中的常见问题

doi:10.7517/issn.1674-0475.180301

影像科学与光化学 ›› 2018, Vol. 36 ›› Issue (6): 532-538.DOI: 10.7517/issn.1674-0475.180301

激光共聚焦显微技术在共定位应用中的常见问题

王娟娟, 魏学红

山西大学大型科学仪器中心, 山西太原 030006

收稿日期:2018-02-27 修回日期:2018-04-27 出版日期:2018-11-15 发布日期:2018-11-15
通讯作者: 王娟娟
基金资助:
2016年中央提升王娟娟人才事业启动经费（304545007）项目资助

Common Problems of Confocal Laser Scanning Microscopy on Colocalization

WANG Juanjuan, WEI Xuehong

Scientific Instrument Center, Shanxi University, Taiyuan 030006, Shanxi, P. R. China

Received:2018-02-27 Revised:2018-04-27 Online:2018-11-15 Published:2018-11-15

摘要/Abstract

摘要： 荧光共定位分析是当今生物显微成像中一个极为常见的技术。本文从以下4个方面详细介绍了使用Zeiss LSM880型激光扫描共聚焦显微镜（confocal laser scanning microscope，CLSM）进行共定位研究时常见的问题及解决方法：1.如何用共聚焦进行共定位实验；2.如何进行共定位分析；3.如何评价共定位；4.如何判定共定位结果。在共定位研究中，只要按照共定位实验的基本要求拍摄图像，并按照上述方法进行分析，便可得到准确可靠的共定位分析结果。

关键词: 激光共聚焦显微技术, 共定位, 评价方法

Abstract: The colocalization of fluorescence is a common technique in the biological imaging. In order to get true results of colocalization, we describe the following four aspects in detail, which are common problems of colocalization by Zeiss LSM880 (confocal laser scanning microscope, CLSM), as well as the solutions. First, how to use CLSM for colocalization experiments. Second, how to conduct colocalization analysis. Third, how to evaluate colocalization. Fourth, how to determine the colocalization results. In the colocalization study, we can get the accurate and reliable colocalization analysis results if we take the images strictly following the basic requirements of the colocalization experiment and analyse them according to the above methods.

Key words: confocal laser scanning microscopy, colocalization, evaluation method

王娟娟, 魏学红. 激光共聚焦显微技术在共定位应用中的常见问题[J]. 影像科学与光化学, 2018, 36(6): 532-538.

WANG Juanjuan, WEI Xuehong. Common Problems of Confocal Laser Scanning Microscopy on Colocalization[J]. Imaging Science and Photochemistry, 2018, 36(6): 532-538.

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激光共聚焦显微技术在共定位应用中的常见问题

Common Problems of Confocal Laser Scanning Microscopy on Colocalization

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