壳聚糖支载菁染料荧光探针及荧光特性

doi:10.7517/j.issn.1674-0475.2009.04.307

影像科学与光化学 ›› 2009, Vol. 27 ›› Issue (4): 307-313.DOI: 10.7517/j.issn.1674-0475.2009.04.307

壳聚糖支载菁染料荧光探针及荧光特性

刘志军, 费学宁, 谷迎春

天津城市建设学院材料科学与工程系, 天津300384

收稿日期:2008-12-01 修回日期:2009-01-07 出版日期:2009-07-23 发布日期:2009-07-23
通讯作者: 费学宁,E-mail:xueningfei@126.com.
基金资助:
国家自然科学基金项目(项目编号:20772090);天津市应用基础及前沿技术研究计划重点项目(项目编号:08JCZDJC18200)。

Study on Fluorescence Characteristics of Chitosan-supported Cyanine Dye Fluorescence Probes

LIU Zhi-jun, FEI Xue-ning, GU Ying-chun

Department of Material Science and Engineering, Tianjin Institute of Urban Construction, Tianjin 300384, P. R. China

Received:2008-12-01 Revised:2009-01-07 Online:2009-07-23 Published:2009-07-23

摘要/Abstract

摘要： 壳聚糖支载有机荧光染料可在生理条件下改变其荧光特性,本文采用壳聚糖(CTS)支载有机荧光染料Cy3,利用红外光谱和热分析对产物进行了表征,研究了壳聚糖支载的荧光染料紫外吸收光谱和荧光光谱特性,结果表明,壳聚糖支载的荧光染料Cy3的紫外吸收特征峰的波长无明显变化,荧光强度明显增强,支载后的荧光染料光稳定性良好,初步细胞标记实验显示,壳聚糖支载后的Cy3染料对脑胶质瘤细胞具有良好的亲和性,并能穿透细胞膜且发出明显荧光。

关键词: Cy3, 壳聚糖支栽, 荧光特征, 紫外吸收, 细胞标记

Abstract: Organic fluorescent Dyes can be supported by Chitosan and the fluorescent Characteristics in physiological environment can be changed.In this paper,unsymmetrical water-soluble cyanine dye(Cy3) was supported by Chitosan(CTS),and the product(Cy3-CTS) was characterized by IR and DTA.The fluorescence Characteristics and ultraviolet absorbance were studied.The results showed that the characteristic peak of UV absorption spectrum of Cy3-CTS shifted little compared to that of Cy3,but the fluorescence intensity enhanced obviously with good photostability.Primary experimental study on labeled cells indicated that Cy3-CTS can pass through the cell membrane with good affinity to glioma cell and show strong fluorescence.

Key words: Cy3, chitosan-supported, fluorescence characteristic, ultraviolet absorption, labeled cells

中图分类号:

TQ57

刘志军, 费学宁, 谷迎春. 壳聚糖支载菁染料荧光探针及荧光特性[J]. 影像科学与光化学, 2009, 27(4): 307-313.

LIU Zhi-jun, FEI Xue-ning, GU Ying-chun. Study on Fluorescence Characteristics of Chitosan-supported Cyanine Dye Fluorescence Probes[J]. Imaging Science and Photochemistry, 2009, 27(4): 307-313.

参考文献

[1] 陈中举,张燕玲,黄金瑛.荧光标记生物大分子及其应用[J].国外医学生物医学工程分册,2004,27(6):348-352.Chen Z J,Zhang Y L,Huang J Y.The labeling of biological big molecule with fluoreseence dye and its applications[J].Foreign Medical Biomedical Engineering Fascicle,2004.27(6):348-352.
[2] 陈秀英,彭孝军.DNA分子荧光探针[J].染料与染色,2004,41(6):315-345.Chen X Y,Peng X J.DNA fluorescence probes[J].Dyestuffs and Coloration,2004,41(6):315-345.
[3] 赵佳胤,邬建敏.壳聚糖纳米粒子荧光探针的制备和表征[J].分析化学,2006,34(11):1555-1559.Zhao J Y,Wu J M.Preparation and characterization of the fluorescent chitosan nanoparticle probe[J].Chinese Journal of Analytical Chemistry,2006,34(11):1555-1559.
[4] Borchard G.Chitosan for gene delivery[J].Adv.Drug Deliv,2001,52:145-150.
[5] Khor E,Lira L Y.Implantable applications of chitin and hitosan[J].Biomaterials,2003,24(13):2339-2349.
[6] 孙成才,霍冀川,雷永林等.近红外吸收功能菁染料的研究进展[J].材料导报,2006,20(8):48-51.Sun C C,Huo J C,Lei Y L,et al.The advance of study on the near-infrared absorbing cyanine dyes[J].Materials Review,2006,20(8):48-51.
[7] 楼开炎,饯旭红,宋恭华.不对称水溶性菁染料的简便制备与纯化方法[J].华东理工大学学报,2002,28(2):212-215.Lou K Y,Qian X H,Song G H.Synthesis and purification of unsymmetrical water-soluble cyanine dyes[J].Journal of East China University of Scigtlce and Technology,2002,28(2):212-215.
[8] Fei X N,Yang S B,zhang B L,Liu Z J,et al.Solid phase synthesis and modification of orange and its derivatives and their spectral propertiea[J].J.Comb.Chem.,2007,9:943-950.
[9] Ye Y P,Sharon B,Jeffery K.Multivalent carbocyanine molecular probes:synthesis and applications[J].Bioconjugate Chem..2005,16,51-61.
[10] 董炎明,王勉,吴玉松等.壳聚糖衍牛物的红外光谱分析[J].纤维素科学与技术,2001,9(2):42-55.Dong Y M,Wang M,Wu Y S.FTIR spectroscopic detemainations of chitosan derivatives[J].Journal of Cellulose Science and Technology,2001,9(2):42-55.

壳聚糖支载菁染料荧光探针及荧光特性

Study on Fluorescence Characteristics of Chitosan-supported Cyanine Dye Fluorescence Probes

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