蔡司Axio Scan.Z1全自动数字玻片扫描系统的应用

doi:10.7517/issn.1674-0475.190302

影像科学与光化学 ›› 2019, Vol. 37 ›› Issue (4): 324-331.DOI: 10.7517/issn.1674-0475.190302

蔡司Axio Scan.Z1全自动数字玻片扫描系统的应用

尹伟¹, 刘双双¹, 郑艳榕², 张翔南²

1. 浙江大学医学院公共技术平台, 浙江杭州 310058;
2. 浙江大学药学院, 浙江杭州 310058

收稿日期:2019-03-27 出版日期:2019-07-15 发布日期:2019-07-15
通讯作者: 张翔南
基金资助:
2019年度浙江省分析测试项目（LGC19H090001）

The Applications of Zeiss Axio Scan.Z1 Automatic Digital Slide Scanning System

YIN Wei¹, LIU Shuangshuang¹, ZHENG Yanrong², ZHANG Xiangnan²

1. The Core Facilities, Zhejiang University School of Medicine, Zhejiang University, Hangzhou 310058, Zhejiang, P. R. China;
2. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, P. R. China

Received:2019-03-27 Online:2019-07-15 Published:2019-07-15

摘要/Abstract

摘要： 全自动切片扫描技术优势大，应用范围广，已逐步成为形态学和病理学研究的一种变革性辅助手段。本文介绍了蔡司Axio Scan.Z1切片扫描系统的配置参数（光学系统、物镜、分辨率、扫描速度、相机等）和成像特点，阐述了蔡司Axio Scan.Z1在大批量全组织扫描、组织病理学、神经生物学等研究领域的重要应用。总结了仪器在使用过程中遇到的问题，分析了原因并提出解决方案，为科研工作提供更好的共享服务。

关键词: 全自动数字玻片扫描技术, 蔡司Axio Scan.Z1, 大批量全组织扫描

Abstract: The full-automatic slice scanning technology has great advantages and wide application scope, and gradually becomes a revolutionary auxiliary means for morphological and pathological research. In this paper, the configuration parameters (optical system, objective lens, resolution, scanning speed, camera, etc.) and imaging characteristics of Zeiss Axio Scan.Z1 slice scanning system are introduced, and the important applications of Zeiss Axio Scan.Z1 in large-scale whole tissue scanning, histopathology, neurobiology and other research fields are expounded. This paper summarizes the problems encountered during the use of the instrument, analyzes the possible causes, and puts forward the solutions to ensure that the instrument provides better sharing services for scientific research.

Key words: full-automatic slice scanning technology, Zeiss Axio Scan.Z1, large-scale whole tissue scanning

尹伟, 刘双双, 郑艳榕, 张翔南. 蔡司Axio Scan.Z1全自动数字玻片扫描系统的应用[J]. 影像科学与光化学, 2019, 37(4): 324-331.

YIN Wei, LIU Shuangshuang, ZHENG Yanrong, ZHANG Xiangnan. The Applications of Zeiss Axio Scan.Z1 Automatic Digital Slide Scanning System[J]. Imaging Science and Photochemistry, 2019, 37(4): 324-331.

参考文献

[1] 张国荣,李丽,王文奇.数字切片在组织学实验教学中的应用[J].实验室研究与探索,2017, 36(6):216-218. Zhang G R, Li L,Wang W Q.Applications of digital slices in histology experiment teaching[J].Laboratory Research and Exploration, 2017, 36(6):216-218.
[2] Arya M, Thrasivoulou C, Henrique R, Millar M, Hamblin R, Davda R, Aare K, Masters J R, Thomson C, Muneer A, Patel H R H, Ahmed A. Targets of Wnt/ß-catenin transcription in penile carcinoma[J].Plos One, 2015, 10(4):e0124395.
[3] Tian L, Goldstein A, Wang H, Ching Lo H, Sun Kim I, Welte T, Sheng K, Dobrolecki LE, Zhang X, Putluri N, Phung T L, Mani S A, Stossi F, Sreekumar A, Mancini M A, Decker W K, Zong C,Lewis M T, Zhang X H. Mutual regulation of tumour vessel normalization and immunostimulatory reprogramming[J]. Nature, 2017, 544(13):250-254.
[4] Saul L, Ilieva K M, Bax H J, Karagiannis P, Correa I, Rodriguez-Hernandez I, Josephs D H, Tosi I, Egbuniwe I U, Lombardi S, Crescioli S, Hobbs C, Villanova F, Cheung A, Geh J L C, Healy C, Harries M, Sanz-Moreno V, Fear D J, Spicer J F, Lacy K E, Nestle F O, Karagiannisa S N. IgG subclass switching and clonal expansion in cutaneous melanoma and normal skin[J].Scientific Reports, 2016, 6:29736.
[5] Doyle W I, Dinser J A, Cansler H L, Zhang X J, Dinh D D, Browder N S, Riddington I M, Meeks J P. Faecal bile acids are natural ligands of the mouse accessory olfactory system[J]. Nature Communications, 2016, 7:11936.
[6] Adil M M, Rodrigues G M, Kulkarni R U, Rao A T, Chernavsky N E, Miller E W, Schaffer D V.Efficient generation of hPSC-derived midbrain dopaminergic neurons in a fully defined, scalable, 3D biomaterial platform[J]. Scientific Reports, 2017, 7:40573.
[7] Cha M,Um S W,Kwon M, Nam T S, Lee B H. Repetitive motor cortex stimulation reinforces the pain modulation circuits of peripheral neuropathic pain[J].Scientific Reports, 2017, 7(1):7986.
[8] Rousseau D, Widiez T, Tommaso S D, Rositi H, Adrien J, Maire E, Langer M, Olivier C, Peyrin F, Rogowsky P. Fast virtual histology using X-ray in-line phase tomography:application to the 3D anatomy of maize developing seeds[J].Plant Methods, 2015, 11:55.
[9] Bin B H,Bhin J,Takaishi M, Toyoshima K E, Kawamata S, Ito K, Hara T, Watanabe T, Irié T, Takagishi T, Lee S H, Jung H S, Rho S, Seo J, Choi D H, Hwang D, Koseki H, Ohara O, Sano S, Tsuji T, Mishima K, Fukada T. Requirement of zinc transporter ZIP10 for epidermal development:Implication of the ZIP10-p63 axis in epithelial homeostasis[J].Proc Natl Acad Sci USA, 2017, 114(46):12243-12248.
[10] Bjarnadóttir K,Benkhoucha M,Merkler D, Weber M S, Payne N L, Bernard C C A, Molnarfi N, Lalive P H. B cell-derived transforming growth factor-β1 expression limits the induction phase of autoimmune neuroinflammation[J]. Scientific Reports, 2016, 6:34594.
[11] Sacchetti C, Bai Y, Stanford S M, Di Benedetto P, Cipriani P, Santelli E, Piera-Velazquez S, Chernitskiy V, Kiosses W B, Ceponis A, Kaestner K H, Boin F, Jimenez S A, Giacomelli R, Zhang Z Y, Bottini N. PTP4A1 promotes TGFβ signaling and fibrosis in systemic sclerosis[J].Nature Communications, 2017, 8:1060.
[12] Bell L C K, Pollara G, Pascoe M, Tomlinson G S, Lehloenya R J, Roe J, Meldau R, Miller R F, Ramsay A, Chain B M, Dheda K, Noursadeghi M. In vivo Molecular Dissection of the Effects of HIV-1 in active tuberculosis[J].PLOS Pathogens, 2016, 12(3):e1005469.
[13] Dyment N A, Breidenbach A P, Schwartz A G, Russell R P, Aschbacher S, Liu H, Hagiwara Y, Jiang R L,Thomopoulos S, Butler D L, Rowe D W. Gdf5 progenitors give rise to fibrocartilage cells that mineralize via hedgehog signaling to form the zonal enthesis[J].Developmental Biology, 2015, 405(1):96-107.
[14] Dyment N A, Jiang X, Chen L, Hong S H, Adams D J, Ackert-Bicknell C, Shin D G, Rowe D W. High-throughput, multi-image cryohistology of mineralized tissues[J].Journal of Visualized Experiments Jove, 2016, 115:e54468.
[15] Lu Q L, Insinna C, Ott C, Stauffer J, Pintado P A, Rahajeng J, Baxa U, Walia V, Cuenca A, Hwang Y S, Daar I O, Lopes S, Lippincott-Schwartz J, Jackson P K, Caplan S, Westlake C J. Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation[J].Nature Cell Biology, 2015, 17(3):228-240.
[16] Zingue D, Weber P, Soltani F, Raoult D, Drancourt M. Automatic microscopic detection of mycobacteria in sputum:a proof-of-concept[J].Scientific Reports, 2018, 8(1):11308.

蔡司Axio Scan.Z1全自动数字玻片扫描系统的应用

The Applications of Zeiss Axio Scan.Z1 Automatic Digital Slide Scanning System

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