基于金纳米棒-壳聚糖复合膜的葡萄糖生物传感器

doi:10.7517/j.issn.1674-0475.2008.04.271

影像科学与光化学 ›› 2008, Vol. 26 ›› Issue (4): 271-279.DOI: 10.7517/j.issn.1674-0475.2008.04.271

基于金纳米棒-壳聚糖复合膜的葡萄糖生物传感器

杜爱铭^1,2, 任湘菱², 唐芳琼², 张琳², 刘奉岭¹

1. 山东师范大学, 化学化工与材料科学学院, 山东, 济南, 250014;
2. 中国科学院, 理化技术研究所, 北京, 100190

收稿日期:2007-12-27 修回日期:2008-03-05 出版日期:2008-07-23 发布日期:2008-07-23
通讯作者: 唐芳琼, Tel:010-82543521, Fax:010-62554670.
基金资助:
国家自然科学基金项目(90406024,60602006,60736001).

Glucose Biosensor Based on the Gold Nanorod/Chitosan Composite Film as Immobilization Matrix

DU Ai-ming^1,2, REN Xiang-ling², TANG Fang-qiong², ZHANG Lin², LIU Feng-ling¹

1. Department of Chemistry, Shandong Normal University, Jinan 250014, Shandong, P.R.China;
2. Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R.China

Received:2007-12-27 Revised:2008-03-05 Online:2008-07-23 Published:2008-07-23

摘要/Abstract

摘要： 本文采用金纳米棒-壳聚糖复合膜固定葡萄糖氧化酶构建电流型葡萄糖生物传感器.通过电化学交流阻抗法和循环伏安法对酶膜状态进行了表征,得到了相应的等效电路和动力学参数.实验结果表明,金纳米棒-壳聚糖复合膜可以辅助电子传递,提高电极的电流响应,并使生物传感器的使用温度范围有很大的扩展.此传感器表现出对葡萄糖溶液浓度的优良响应,线性范围在2.78×10^-5mol/L—2.22×10^-3mol/L,响应灵敏度约为7.819μA·cm^-2(mmol/L)^-1,表观米氏常数为10mmol/L.本工作还研究了温度和溶液pH值对电极电流响应的影响.

关键词: 金纳米棒, 壳聚糖, 葡萄糖氧化酶, 生物传感器, 电化学阻抗谱

Abstract: Au nanorod /chitosan have good biocompatibility,and are suitable for enzyme immobilization.In this paper,Au nanorod /chitosan composite matrix was developed to fabricate the glucose biosensor.The fabrication of electrode is simple and easily operated.The enzyme electrode was characterized by electrochemistry impedance spectrum and cyclic voltammetry,and the equivalent circuit was given.The experimental results indicated that the Au nanorod/chitosan composite membrane could increase the electronic transfer and response current of electrode.The linear range was 2.78×10^-5 to 2.22×10^-3 mol/L with a detection limit of 5.0×10-6 mol/L at 3 σ and a sensitivity of 7.819 μA·cm^-2(mmol/L)^-1,the apparent Michaelis-Menten constant(K_m) is 10.387 mmol/L.The current response maintained 80% of original response after the electrode was repeatedly used 50 times.Further,it was found that 35 ℃ and 6.8 of pH are the set of optimal for the electrode measurement.

Key words: Au nanorod, chitosan, glucose oxidase, biosensor, electrochemistry impedance spectrum

中图分类号:

杜爱铭, 任湘菱, 唐芳琼, 张琳, 刘奉岭. 基于金纳米棒-壳聚糖复合膜的葡萄糖生物传感器[J]. 影像科学与光化学, 2008, 26(4): 271-279.

DU Ai-ming, REN Xiang-ling, TANG Fang-qiong, ZHANG Lin, LIU Feng-ling. Glucose Biosensor Based on the Gold Nanorod/Chitosan Composite Film as Immobilization Matrix[J]. Imaging Science and Photochemistry, 2008, 26(4): 271-279.

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基于金纳米棒-壳聚糖复合膜的葡萄糖生物传感器

Glucose Biosensor Based on the Gold Nanorod/Chitosan Composite Film as Immobilization Matrix

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