Label-free and Rapid Detection of ATP Based on Molybdenum Oxide Quantum Dots

doi:10.7517/issn.1674-0475.200602

Abstract

Abstract: Molybdenum oxide quantum dots (MoO_x QDs) with excellent fluorescence performance were synthesized using MoS₂ and H₂O₂ as the raw materials by a one-pot method at the room temperature. Based on the fluorescence "off-on" mode, a new rapid fluorescence detection of ATP was constructed, in which MoO_x QDs was a fluorescent probe and Cu²⁺ was a quencher. Under the optimized condition, pH 7.5, 0.051 mg/mL MoO_x QDs and 50 mol/L Cu²⁺, the fluorescence at 520 nm was gradually enhanced with increasing ATP concentration in the range of 25-250 mol/L. The calibration curve was F=785.40+9.05c with the correlation coefficient of 0.993. The coexistence of BSA, Ni²⁺, Mn²⁺, Mg²⁺, Fe³⁺, Co²⁺ and Ca²⁺ has negligible effect on ATP detection. Therefore, the "off-on" detection platform shows rapid and stable for sensing for ATP.

Key words: adenosine triphosphate, MoO_x quantum dots, Cu²⁺, fluorescent probe

HUANG Haiqing, XIAO Saijin, YANG Guangzhaoyao, ZHAO Jiahui. Label-free and Rapid Detection of ATP Based on Molybdenum Oxide Quantum Dots[J]. Imaging Science and Photochemistry, 2020, 38(6): 950-955.

References

[1] Gourine A V, Llaudet E, Dale N, et al. ATP is a mediator of chemosensory transduction in the central nervous system[J]. Nature, 2005, 436(7047):108-111.
[2] Lian Y K, Jiang H, Feng J Z, et al.Direct and simultaneous quantification of ATP, ADP and AMP by 1H and 31P nuclear magnetic resonance spectroscopy[J]. Talanta, 2016,150:485-492.
[3] Cheng J H, Sun D W, Zeng X A, et al. Recent advances in methods and techniques for freshness quality determination and evaluation of fish and fish fillets:a review[J].Critical Reviews in Food Science & Nutrition, 2015, 55(7):1012-1225.
[4] Pistelok F, Pohl A, Stuczynski T, et al. Strengths and weaknesses in the determination of Saccharomyces cerevisiae cell viability by ATP-based bioluminescence assay[J]. Ecological Chemistry & Engineering S, 2016, 23(2):259-270.
[5] Han T, Wren M, Dubois K, et al. Application of ATP-based bioluminescence for bioaerosol quantification:effect of sampling method[J].Journal of Aerosol Science, 2015,90:114-123.
[6] 周春喜,郁荣,吕海燕,等. 三磷酸腺苷、二磷酸腺苷和一磷酸腺苷的毛细管电泳分析研究[J].分析科学学报, 2003,(5):433-434.
[7] Liebers V, Bachmann D, Franke G, et al. Determination of ATP-activity as a useful tool for monitoring microbial load in aqueous humidifier samples[J]. International Journal of Hygiene and Environmental Health, 2015, 218(2):246-253.
[8] Zhou L, Xue X X, Zhou J Hi, et al. Fast determination of adenosine 5'-triphosphate (ATP) and its catabolites in royal jelly using ultraperformance liquid chromatography[J]. Journal of Agricultural and Food Chemistry, 2012, 60(36):8994-8999.
[9] Wu H Q, Wu Q P, Zhang J M, et al. Study on rapid quantitative detection of total bacterial counts by the ATP-bioluminescence and application in probiotic products[J]. International Journal of Food Science and Technology, 2011, 46(5):921-929.
[10] Srivastava P, Razi S S, Ali R, et al. Highly sensitive cell im-aging "off-on" fluorescent probe for mitochondria and ATP[J].Biosensors and Bioelectronics, 2015, 69:179-185.
[11] Wang W, Zhao N, Li X X, et al. Isothermal amplified detection of ATP using Au nanocages capped with a DNA molecular gate and its application in cell lysates[J]. The Analyst, 2015,140(5):1672-1677.
[12] Tedsana W, Tuntulani T, Ngeontae W. A highly selective turn-on ATP fluorescence sensor based on unmodified cysteamine capped CdS quantum dots[J]. Analytica Chimica Acta, 2013, 783(11):65-73.
[13] Shi F P, Li Y, Lin Z H, et al. A novel fluorescent probe for adenosine 5-triphosphate detection based on Zn²⁺-modulated l-cysteine capped CdTe quantum dots[J]. Sensors and Actuators B:Chemical, 2015, 220:433-440.
[14] Derfus A M, Chan W C W, Bhatia S N. Probing the cytotoxicity of semiconductor quantum dots[J]. Nano Letters, 2004, 4(1):11-18.
[15] 胡先运,江家志,王传明,等. 氧化石墨烯/适配体-量子点荧光探针用于ATP检测[J].分析试验室, 2018, 37(9):1003-1007.
[16] Xiong Y, Cheng Y, Wang L, et al. An "off-on" phosphorescent aptasensor switch for the detection of ATP[J]. Talanta, 2018, 190:226-234.
[17] Anh Tran T, Krishnamoorthy K, Song Y W, et al.Toxicity of nano molybdenum trioxide toward invasive breast cancer cells[J]. ACS Applied Materials & Interface, 2014, 6(4):2980-2986.
[18] Krishnamoorthy K, Premanathan M, Veerapandian M, et al. Nanostructured molybdenum oxide-based antibacterial paint:effective growth inhibition of various pathogenic bacteria[J]. Nanotechnology, 2014, 25(31):596-606.
[19] Illyaskutty N, Sreedhar S, Kumar G S, et al. Alteration of architecture of MoO₃ nanostructures on arbitrary substrates:growth kinetics, spectroscopic and gas sensing properties[J]. Nanoscale, 2014, 6:13882-13894.
[20] Wang Y X, Zhang X, Luo Z M, et al. Liquid-phase growth of platinum nanoparticles on molybdenum trioxide nanosheets:an enhanced catalyst with intrinsic peroxidase-like catalytic activity[J]. Nanoscale, 2014, 6(21):12340-12344.
[21] Yi Q H, Zhai P F, Sun Y H, et al. Aqueous solution-deposited molybdenum oxide films as an anode interfacial layer for organic solar cells[J]. ACS Applied Materials & Interfaces, 2015, 7:18218-18224.
[22] Xiao S J, Zhao X J, Chu Z J, et al. New off-on sensor for captopril sensing based on photoluminescent MoO_x quantum Dots[J]. ACS Omega, 2017, 2:1666-1671.
[23] Xiao S J, Zhao X J, Chu Z J, et al. Highly photoluminescent molybdenum oxide quantum dots:one pot synthesis and application in 2,4,6-trinitrotoluene determination[J].ACS Applied Materials & Interfaces, 2016, 8(12):8184-8191.