Construction of Dysprosium Fluorescent Probe and Application of Determination on Tetracrycline Hydrochlorid Residues in Milk

doi:10.7517/j.issn.1674-0475.2014.06.565

Abstract

Abstract:

A novel method for the detection of tetracycline was described on the basis of ternary dysprosium fluorescent probe. The tetracycline hydrochloride has flurescence quenching effect for dysprosium fluorescent probe in ethonal system. The excitation wavelength of the system was at 305 nm and the emission wavelength at 574 nm, respectively. The optimized condition were measured: the best ration of dysprosium (Dy³⁺), sulfosalicylic acid (SSA), Tri-n-octyl phosphine oxide (TOPO) was 1:2:0.1, and the best fluorescence detection time was 30 min. Moreover, the calibration curve of detection for tetracycline hydrochloride was established which ranged from 1×10^-6 to 2×10^-5 mol/L. The dysprosium fluorescent probe was utilized to conduct the sample recovery test of the treated milk samples, the recovery of the milk sample test was 96.9 %104.4 %. As a result, the experimental research demonstrated that the method constructed is scientific and feasible, and has high selectivity to the tetracycline hydrochloride.

Key words: dysprosium, fluorescent probe, hydrochloric acid tetracycline, milk

WANG Manli, LI Xin, ZHANG Xiaohui, MING Huami, YIN Hongzong, XU Kun. Construction of Dysprosium Fluorescent Probe and Application of Determination on Tetracrycline Hydrochlorid Residues in Milk[J]. Imaging Science and Photochemistry, 2014, 32(6): 565-571.

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