Progress of Two-photon Polymerization Technology in Tissue Engineering

doi:10.7517/j.issn.1674-0475.2017.04.005

Abstract

Abstract:

The natural extracellular matrix (ECM) is a collection of extracellular macromolecules distributed on the cell surfaces or in the intercellular spaces, having a complex grid structure. Through the intimate interaction with cells, it regulates cell growth, proliferation and differentiation. Up to now, the cell-ECM interaction has not been fully understood. The new-emerging tissue engineering involves the construction of a scaffold (ECM in vitro) for the formation of new tissue or organ, which is beneficial to deeply understand the cell-ECM interaction and further lay the foundation for the development of regenerative medicine. Two-photon polymerization (2PP) is an ideal way to construct ECM in vitro due to its advantages of large penetration depth, high spatial selectivity and little damage to biological tissue. In this review, the principles of two-photon absorption and 2PP are introduced firstly. The development of water-soluble photosensitizers and photo-polymerizable hydrogels for tissue engineering are mainly summarized. Finally, the perspective of 2PP technology for tissue engineering is also proposed.

Key words: two-photon polymerization(2PP), tissue engineering, water-soluble photosensitizer, hydrogels

HUANG Xing, LIU Yuan, WANG Xiaopu, ZHAO Yuxia. Progress of Two-photon Polymerization Technology in Tissue Engineering[J]. Imaging Science and Photochemistry, 2017, 35(4): 396-412.

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