Effect of Acid Pretreatment on IEP and Protein Patterns of Gelatin from the Bovine Bones by Protease-Conditioned Process

doi:10.7517/j.issn.1674-0475.2012.02.102

Abstract

Abstract: Gelatins were prepared from the bone pretreated using hydrochloride with various concentrations by protease-conditioned method in this article. The yields of protease-conditioned gelatin were decreased as the concentration of HCl was increased from 0 to 4.5% (mass fraction). The bloom values of protease-condition gelatin were all higher than 300 when the bovine bone were pretreated with HCl. Compared with commercial type B gelatin, protease-conditioned gelatin exhibited higher bloom strength, lower viscosity and higher IEP value. Compared with protein patterns of type B gelatin, different protein patterns of protease-conditioned gelatin were noticeable with the immersing of the new bands at the Mw of 165 KDa and 120 KDa. The IEPs of protease-conditioned gelatin were all in alkaline range which was related to the content of arginine. The results showed that the pretreatment process of bone was very important for protease-conditioned gelatin because the yield, viscosity and IEP were determined by the pretreatment condition.

Key words: gelatin, protease-conditioned, acid pretreatment, IEP, protein patterns

CLC Number:

ZHANG Bing, SHI Jing-jing, WANG Ying, GUO Yan-chuan, CHEN Li-juan. Effect of Acid Pretreatment on IEP and Protein Patterns of Gelatin from the Bovine Bones by Protease-Conditioned Process[J]. Imaging Science and Photochemistry, 2012, 30(2): 102-109.

References

[1] Karim A,Rajeev B.Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins[J].Food Hydrocolloids,2009,23:567-576.
[2] Olsen D,Yang C,Bodo M,et al. Recombinant collagen and gelatin for drug delivery[J].Adv. Drug Delivery Rev.,2003,55(12):1547-1567.
[3] Reinhard S,Herbert G.Gelatin Handbook:Theory and Industrial Practice[M].Weinheim: WILEY-VCH Press. 2007.1-36.
[4] 缪进康.明胶及其在科技领域中的利用[J].明胶科学与技术,2009,29(1):28-49. Miu J K, Gelatin and its application in the field of science and technology[J]. The Science and Technology of Gelatin, 2009,29(1):28-49.
[5] 姚龙坤.明胶在食品工业中的应用简述[J].明胶科学与技术,2009,29(1):24-25. Yao L K. Introduction of gelatin in food application[J]. The Science and Technology of Gelatin,2009,29(1):24-25.
[6] Badii F,Howell N.Fish gelatin: structure, gelling properties and interaction with egg albumen proteins[J].Food Hydrocolloids,2006,20(5):630-640.
[7] Zhang F,Xu S,Wang Z.Pre-treatment optimization and properties of gelatin from freshwater fish scales [J].Food and Bioproducts Processing,2010,doi:10.1016/j.fbp.2010.05.003.
[8] Chomarat N,Robert L,Seris J,et al.Comparative efficiency of pepsin and proctase for the preparation of bovine skin gelatin[J].Enzyme and Microbial Technology,1994,16(9):756-760.
[9] Cannan R,Muntwyler E.The action of pepsin on gelatin[J].Biochemical Journal,1930, 24(4):1012-1020.
[10] 陈丽娟,等.酶降解骨胶原制备明胶的方法.中国专利,ZL02 125 447.8,2005-4-13. Chen L J. Method of extracting gelatin using enzymatic processing technology from bone collogen. Chinese patent, ZL02 125 447.8,2005-4-13.
[11] 张锋,施辉阳,张鹏.酶法制备明胶的新工艺研究[J].北京化工大学学报,2003,30(1):9-12. Zhang F, Shi H Y, Zhang P. A new process for preparation of gelatin with enzyme[J]. Journal of Beijing University of Chemical Technology, 2003, 30(1): 9-12.
[12] 陈丽娟,王颖,彭必先.胰蛋白酶降解骨胶原与明胶组分含量之间的关系[J].感光科学与光化学,1998,16(3):258-263. Chen L J, Wang Y, Peng B X. The relationship between the trypsin digestion of bone collagen and the component content of gelatin[J]. Photographic Science and Photochemistry, 1998, 16(3), 258-263.
[13] Elharfaoui N,Djabourov M,Babel W.Molecular weight influence on gelatin gels:structure,enthalpy and rheology[J].Macromolecular Symposia,2007,256(1):149-157.
[14] Norziah M,Al-Hassan A,Khairulnizam A,et al.Characterization of fish gelatin from surimi processing wastes: Thermal analysis and effect of transglutaminase on gel properties[J].Food Hydrocolloids,2009,23(6):1610-1616.
[15] Jonhard E,Ingvild J,Ann-Sissel U,et al.Mechanical properties of mammalian and fish gelatins based on their weight average molecular weight and molecular weight distribution[J].Food Hydrocolloids,2009,23:2315-2321.
[16] Jongjareonrak A,Rawdkuen S,Chaijan M,et al.Chemical compositions and characterisation of skin gelatin from farmed giant catfish (Pangasianodon gigas) [J].LWT - Food Science and Technology,2010,43(1):161-165.
[17] Eastoe J, Leach A. Chemical constitution of gelatin. In A. C. A.G. Ward (Ed.), The Science and Technology of Gelatin[M]. London: Academic Press. 1977.