A FRET Fluorescent Probe for α-Ketoglutaric Acid Based on Rhodamine and BODIPY

doi:10.7517/j.issn.1674-0475.2017.03.265

Abstract

Abstract:

Small molecule α-ketoglutaric acid (α-KA) is a very important product of metabolism in human body, which exhibits direct or indirect relation with many kinds of diseases. A fluorescence resonance energy transfer (FRET) ratiometric probe for α-KA containging rhodamine and BODIPY has been synthesized. As the energy donor, the fluorescence spectrum of BODIPY matches well with the absorption spectrum of rhodamine which is chosen as the energy acceptor. Research shows that the orginal fluorescence spectrum of probe RDM-BO is similar with BODIPY. While the α-KA induces ring-open reacntion with rhodamine unit of RDM-BO, the molecular conjugate is changed, resulting in new fluorescence peaks. The detection limit could reach 3.14 μmol·L^-1.

Key words: rhodamine, BODIPY, α-ketoglutaric acid, fluroescence probe

HE Ye, TANG Junma, GUO Zhiqian, JIN Pengwei, ZHU Shiqin, ZHU Weihong. A FRET Fluorescent Probe for α-Ketoglutaric Acid Based on Rhodamine and BODIPY[J]. Imaging Science and Photochemistry, 2017, 35(3): 265-273.

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