A BODIPY-based Fluorescent Probe for Selective Detection of GSH

doi:10.7517/j.issn.1674-0475.2017.04.016

Abstract

Abstract:

Here, a BODIPY-based dye was constructed by appending malononitrile, an electron acceptor, to the BODIPY core via a vinylene bridge. The designed BODIPY proceeded aromatic nucleophilic substitution (S_NAr) toward sulfhydryl-containing nucleophiles in buffer systems. Experimental data demonstrated that the reactions between CN-B-Cl and thiol-containing amino acids (GSH, Cys/Hcy) produced different BODIPYs with distinct fluorescent signals, thus allowing CN-B-Cl for discriminating GSH from Cys/Hcy.

Key words: BODIPY, aromatic nucleophilic substitution, fluorescent probe, GSH

FEI Qiang, AN Jiancai, ZHAO Chunchang. A BODIPY-based Fluorescent Probe for Selective Detection of GSH[J]. Imaging Science and Photochemistry, 2017, 35(4): 546-551.

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