Electrochemical Selective Recognition of Tryptophan Enantiomers Based on Graphene/Chitosan Composite Films

doi:10.7517/issn.1674-0475.180402

Abstract

Abstract: In this research, graphene/chitosan composite films were designed and used as electrode for electrochemical enantioselective recognition of tryptophan (Trp) enantiomers. The structure of the graphene/chitosan composite films were characterized using X-ray diffraction, FT-IR spectroscopy, Raman spectroscopy, XPS spectroscopy, transmission and scanning electron microscopy. Trp enantiomers were recognized using graphene/chitosan composite electrode by a differential pulse voltammetry. Experimental data indicated that L-Trp exhibited stronger electrochemical response than D-Trp, and that a linear relationship between the current density of the oxidative peak and the concentrations of L-/D-Trp was established in a concentration span ranging from 0.3×10^-4 mol·L^-1 to 1.5×10^-4 mol·L^-1. The composite electrode had a number of advantages including controllable thickness and area, good stability, and enhanced electrochemical signals. Therefore, the graphene/chitosan composite electrodes are expected to be used for enantioselective recognition in areas such as molecular drugs and clinical medicine.

Key words: graphene, chitosan, differential pulse voltammetry, amino acids, enantiomeric recognition

ZHANG Jinping, FAN Jingbiao, HUANG Yan, MI Hongyu, GENG Jianxin. Electrochemical Selective Recognition of Tryptophan Enantiomers Based on Graphene/Chitosan Composite Films[J]. Imaging Science and Photochemistry, 2018, 36(5): 398-407.

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