Imaging Science and Photochemistry ›› 2018, Vol. 36 ›› Issue (6): 532-538.DOI: 10.7517/issn.1674-0475.180301

Previous Articles     Next Articles

Common Problems of Confocal Laser Scanning Microscopy on Colocalization

WANG Juanjuan, WEI Xuehong   

  1. Scientific Instrument Center, Shanxi University, Taiyuan 030006, Shanxi, P. R. China
  • Received:2018-02-27 Revised:2018-04-27 Online:2018-11-15 Published:2018-11-15

Abstract: The colocalization of fluorescence is a common technique in the biological imaging. In order to get true results of colocalization, we describe the following four aspects in detail, which are common problems of colocalization by Zeiss LSM880 (confocal laser scanning microscope, CLSM), as well as the solutions. First, how to use CLSM for colocalization experiments. Second, how to conduct colocalization analysis. Third, how to evaluate colocalization. Fourth, how to determine the colocalization results. In the colocalization study, we can get the accurate and reliable colocalization analysis results if we take the images strictly following the basic requirements of the colocalization experiment and analyse them according to the above methods.

Key words: confocal laser scanning microscopy, colocalization, evaluation method