Study on the Determination of Bovine Serum Albumin in Rat Plasma by Synchronous Fluorescence Spectrometry

doi:10.7517/j.issn.1674-0475.2010.05.354

Imaging Science and Photochemistry ›› 2010, Vol. 28 ›› Issue (5): 354-361.DOI: 10.7517/j.issn.1674-0475.2010.05.354

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Study on the Determination of Bovine Serum Albumin in Rat Plasma by Synchronous Fluorescence Spectrometry

QIN Guang-jiong^1,2, GAO Yun-hua¹

1. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
2. Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China

Received:2010-05-11 Revised:2010-05-25 Online:2010-09-23 Published:2010-09-23

Abstract

Abstract: With fluorescein isothiocyanate labeled bovine serum albumin(FITC-BSA) as a model drug,the concentration of FITC-BSA in rat plasma was assayed after tail vein injection by utilizing synchronous fluorescence spectrometry.The impacts of sample pretreatment,measurement parameters were investigated.A simple and effective determination method for exogenous protein in vivo was established by utilizing synchronous spectrometry,whose results match with results by standard enzyme-linked immunosorbent assay(ELISA) very well.

Key words: synchronous fluorescence, bovine serum albumin, enzyme-linked immunosorbent assay

CLC Number:

O657

QIN Guang-jiong, GAO Yun-hua. Study on the Determination of Bovine Serum Albumin in Rat Plasma by Synchronous Fluorescence Spectrometry[J]. Imaging Science and Photochemistry, 2010, 28(5): 354-361.

References

[1] Hopkins A L,Groom C R.The druggable genome[J].Nat.Rev.Drug Disc.,2002,1(9):727-730.
[2] Drews J.Drug discovery:a historical perspective[J].Science,2000,287(5460):1960-1964.
[3] Torchilin V P,Lukyanov A N.Peptide and protein drug delivery to and into tumors:challenges and solutions[J].Drug Discov.Today,2003,8(6):259-266.
[4] Engvall E.Enzyme immunoassay ELISA and EMIT[J].Methods Enzymol.,1980,70(A):419.
[5] 李高.稳定同位系标记药物在临床药代动力学研究中的应用[J].中国临床药理学杂志,2000,16(1):58-62.Li G.The application of stable isotope labelled drugs in clinical pharmacokinetic study[J].Chin.J.Clin.Pharmacol.,2000,16(1):58-62.
[6] Meager A.Biological assays for interferons[J].J.Immunol.Methods,2002,261(1):21-36.
[7] 张琪,王广基.蛋白多肽类药物药代动力学分析方法研究进展[J].药物生物技术,2000,7(2):126-128.Zhang Q,Wang G J.The progress of the analysis method of protein polypeptide drug pharmacokinetics[J].Pharm.Biotechnol.,2000,7(2):126-128.
[8] Woodnutt G,Berry V.Two pharmacodynamic models for assessing the efficacy of amoxicillin-clavulanate against experimental respiratory tract infections caused by strains of streptococcus pneumoniae[J].Antimicrob.Agents Ch.,1999,43(1):29.
[9] Koopmans S J,Wee-Pals L V D,Lowik C,et al.Use of a rat model for the simultaneous assessment of pharmacokinetic and pharmacodynamic aspects of bisphosphonate treatment:Application to the study of intravenous ¹⁴C-labeled 1-hydroxy-3-(1-pyrrolidinyl)-propylidene-1,1-bisphosphonate[J].J.Bone Miner.Res.,2009,9(2):241-246.
[10] Weiss H R.Measurement of cerebral capillary perfusion with a fluorescent label[J].Microvasc.Res.,1988,36(2):172-180.
[11] Cabaniss S E.Synchronous fluorescence spectra of metal-fulvic acid complexes[J].Environ.Sci.Technol.,1992,26(6):1133-1139.
[12] Patra D,Mishra A K.Recent developments in multi-component synchronous fluorescence scan analysis[J].TrAC-Trend.Anal.Chem.,2002,21(12):787-798.
[13] Lorenz J,Gruenstein E.A simple,nonradioactive method for evaluating single-nephron filtration rate using FITC-inulin[J].Am.J.Physiol.-Renal Physiol.,1999,276(1):F172-F177.

Study on the Determination of Bovine Serum Albumin in Rat Plasma by Synchronous Fluorescence Spectrometry

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