Imaging Science and Photochemistry ›› 2014, Vol. 32 ›› Issue (1): 106-112.DOI: 10.7517/j.issn.1674-0475.2014.01.106

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Cyanine-based Near-infrared Ratiometric Fluorescent Probe for Thiols

ZHU Dongjian1, JIANG Hua1,2   

  1. 1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
    2. College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China
  • Received:2013-12-05 Revised:2013-12-17 Online:2014-01-15 Published:2014-01-15

Abstract:

The article describes the design and synthesis of a near-infrared ratiometric fluorescent probe CySS for thiols, the probe CySS has heptamethine cyanine as the reporter, a disulfide group as the thiol reaction site. The properties and application of CySS were studied in detail. The results demonstrate that CySS displays high sensitivity, good selectivity, and pH-independent. Moreover, we have demonstrated that CySS is suitable for imaging thiols in living cells, providing a potentially powerful tool for thiols detection in biological systems.

Key words: thiols, cyanine, near-infrared, ratiometric, fluorescent probe