Imaging Science and Photochemistry ›› 2016, Vol. 34 ›› Issue (4): 346-354.DOI: 10.7517/j.issn.1674-0475.2016.04.346

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A Simple Fluorescent Method for Detection of Mitomycin Based on CdSe/ZnS Quantum Dots

GUO Yu'an, ZHANG Zhifeng, SHEN Chenfan, YAN Guiqin   

  1. School of Life Science, Shanxi Normal University, Linfen 041000, Shanxi, P.R.China
  • Received:2016-04-18 Revised:2016-05-20 Online:2016-07-15 Published:2016-07-15

Abstract:

In this study, we developed a simple and readily implemented fluorescent method for detection of mitomycin based on the effects of CdSe/ZnS quantum dots. At pH 7.4 and reaction time of 10 min, the fluorescence of the CdSe/ZnS quantum dots was quenched by increased concentrations of mitomycin. We used these observations to establish a linear relationship between the quenched fluorescence signal and the mitomycin concentration in the range of 1.8~60 μmol/L, with a detection limit of 0.11 μmol/L, and an correlation coefficient of 0.996. The proposed method is relatively free of interference from coexisting substances, and was shown here to be fully applicable to the detection of mitomycin in mitomycin injection and urine samples.

Key words: CdSe/ZnS quantum dots, fluorescence, mitomycin, detection