Determination of Indole Acetic Acid by SPR Biosensor Based on the Metal Nanoparticles Sensitization

doi:10.7517/j.issn.1674-0475.2017.05.008

Abstract

Abstract:

Surface plasmon resonance (SPR) biosensor was a new method for detecting 3-indole acetic acid (IAA). We adopted two kinds of indole acetic acid SPR biosensors to detect IAA:Traditional sensor 1 and Au/Ag alloy nanoparticles based sensor 2. Sensor 1:The wavenumber shifts were linearly proportional to the IAA concentration in a range from 175 to 350 μg/L with a detection limit of 25 μg/L (S/N=3). Sensor 2:The wavenumber shifts were linearly proportional to the IAA concentration in a range from 17.5 to 250 μg/L with a detection limit of 2.2 μg/L (S/N=3). The results showed that the sensor 2 based on Au/Ag alloy nanoparticles had higher sensitivity and lower detection limit than the the sensor 1. The rate of standard addition recovery was 96%-100.2%, the average was 98.4%. The prepared SPR biosensor had good precision, stability, repeatability and specificity.

Key words: SPR biosensor, indole acetic acid (IAA), IAA antibody, Au/Ag alloy nanoparticles

GAO Wenhui, WANG Zhaoqun, PAN Junqing, YIN Hongzong. Determination of Indole Acetic Acid by SPR Biosensor Based on the Metal Nanoparticles Sensitization[J]. Imaging Science and Photochemistry, 2017, 35(6): 843-852.

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