Application of CdTe QDs-TMPyP Nanocomplex in Imaging of Tumor Cells

doi:10.7517/j.issn.1674-0475.2017.05.011

Abstract

Abstract:

The solution CdTe quantum dots (Dots) with high quantum yield and good homogeneous distribution conjugate meso-tetrakis(N-methylpyridinium-4-yl) porphyrin (TMPyP) to construct QDs-TMPyP nanocomplex. The QDs-TMPyP nanocomplex was achieved to detection of DNA by photo induced electron-transfer (PIET) process. CdTe QDs and CdTe QDs-TMPyP showed a remarkable inhibition for the proliferation of cancer cells in vitro at higher concentrations, but only small effects at lower concentrations. The cells viabilities of HeLa cells were above 92% under treated with lower than 1.0 μmol/L CdTe QDs and CdTe QDs-TMPyP for 24 h. The HeLa cancer cells treated with 0.2 μmol/L CdTe QDs-TMPyP were found to contain Cd and Te atoms by EDS analysis. The uptake of CdTe QDs-TMPyP was easier than CdTe QDs in HeLa cancer cells. The CdTe QDs-TMPyP nanocomplex was successfully achieved in HeLa cells fluorescent imaging, which lays the foundation for the drug delivery and cell imaging.

Key words: quantum dots, TMPyP, fluorescent imaging, DNA

HU Xianyun, MENG Tiehong, HUANG Xinghong, ZHANG Ruguo, JIANG Jiazhi, LIU Ying, ZHU Nan. Application of CdTe QDs-TMPyP Nanocomplex in Imaging of Tumor Cells[J]. Imaging Science and Photochemistry, 2017, 35(6): 853-860.

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