Nanodiamonds Raman Probes for Visualizing the Interaction between Antimicrobial Peptide and Bacteria

doi:10.7517/j.issn.1674-0475.2018.01.009

Abstract

Abstract: This article realizes the application of nanodiamonds (NDs) Raman probes for vital activity observation in bacteria system. The antimicrobial peptide thanatin was immobilized on 100 nm NDs, and the interaction of the NDs-thanatin conjugate with Bacillus subtilis was visualized via Raman imaging with the intrinsic diamond Raman signal (1332 cm^-1) as label. Scanning electron microscope was further utilized to verify the practicability of Raman mapping. Besides, antibacterial activity of NDs-thanatin conjugate was validated, suggesting the adsorbed thanatin on NDs remained superior capability. The results showed the feasibility of nanodiamonds as Raman probes to visualize the bacteria activity, indicating the potential use of NDs in bio-imaging applications.

Key words: nanodiamonds, Raman probes, antimicrobial peptide, antibacterial activity, visualization

LI Dandan, CHEN Xin, WANG Hong, FU Yang, YU Yuan, ZHI Jinfang. Nanodiamonds Raman Probes for Visualizing the Interaction between Antimicrobial Peptide and Bacteria[J]. Imaging Science and Photochemistry, 2018, 36(1): 89-99.

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