Effect and Mechanism of Photo-induced Hemoglobin Kinetics Reaction with Free Cysteine

doi:10.7517/j.issn.1674-0475.2018.02.004

Abstract

Abstract: Hemoglobin (Hb) is a respiratory protein with cyclic conjugated structure, which can absorb light at specific wavelengths and influence the expression of hemoglobin by the structural changes. It was found that the ultraviolet light irradiation could influence the structural changes of Hb, and added free cysteine contributes to the structure stability of methemoglobin (metHb) in irradiation experiment. Irradiated by 266 nm laser,metHb, deoxyhemoglobin(deoxyHb), oxyhemoglobin(HbO₂) and carboxyhemoglobin(HbCO) are excited to their excited states, and the decay processes to the ground state indicated with their corresponding Soret band peak have almost the same time. After adding free cysteine, the electron of cysteine can directly or indirectly transfer to iron porphyrin with the variation of the decay time of excited state iron porphyrin. The significant prolongations of decay time are observed on the photo-irradiated metHb, HbO₂ and HbCO, while slight influences are detected on the photo-irradiated deoxyHb. According to transient absorption spectroscopy, kinetic curves and UV-Vis spectra, while the four forms of hemoglobin are excited to the excited state, their iron porphyrin is reacted to the state of six-coordinated planar structure with one empty coordination position.

Key words: hemoglobin, photo-induced, laser flash photolysis, cysteine, electron transfer

CAO Hongyu, SHI Fei, TANG Qian, WANG Lihao, ZHENG Xuefang. Effect and Mechanism of Photo-induced Hemoglobin Kinetics Reaction with Free Cysteine[J]. Imaging Science and Photochemistry, 2018, 36(2): 149-160.

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