Imaging Science and Photochemistry ›› 2020, Vol. 38 ›› Issue (6): 941-949.DOI: 10.7517/issn.1674-0475.200514

• Review and Articles • Previous Articles     Next Articles

Biomimetic Synthesis of Au QDs and the Application of Glucose Detection in Serum

ZHU Tao1, ZHANG Yan2, DONG Lulu1,3, GUO Pengyue1, YANG Dongzhi1   

  1. 1. Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou 221004, Jiangsu, P. R. China;
    2. Hospital 904 of China Joint Logistic Support Force, Changzhou 213000, Jiangsu, P. R. China;
    3. The First People's Hospital of Changzhou, Changzhou 213000, Jiangsu, P. R. China
  • Received:2020-05-21 Online:2020-11-15 Published:2020-11-15
  • Supported by:
    Xuzhou Natural Science Foundation (KC18201, KC18108,KC19066), and Jiangsu Undergraduate Innovation and Entrepreneurship Training Program (201910313012Z)

Abstract: In the research of new synthesis methods of non-toxic nanomaterials, biomimetic synthesis has attracted the attention of many researchers because of its obvious advantages over chemical synthesis. Herein gold quantum dots (Au QDs) were prepared in living cells driven by cell life metabolism. The characterization shows that the prepared gold Au QDs have cubic crystal structure, uniform size distribution and a diameter of about 20 nm. Cell experiments showed that the biocompatibility of Au QDs synthesized in cells was significantly higher than that of traditional chemical synthesis. Under certain conditions, the fluorescence of Au QDs can be quenched by glucose, and the fluorescence quenching efficiency of Au QDs shows a good linear relationship with glucose concentration, with a linear range of 7.85×10-6-2.5×10-4 mol/L and a detection limit of 3.1×10-8 mol/L. This method was applied to detect blood glucose concentration in diabetic rats, and the result was consistent with that of blood glucose meter. The prepared Au QDs have low cytotoxicity and potential detection ability, and can be used for the detection of small biological molecules.

Key words: biomimetic synthesis, gold quantum dots, cancer cell, glucose