A SPECTROSCOPY STUDY OF THE MIXED LANGMUIR BLODGETT FILMS OF ALKYL GLYCOSIDE WITHPOSITIVE CHARGE AND GLUCOSE OXIDASE

doi:10.7517/j.issn.1674-0475.1998.01.32

Abstract

Abstract: Alkyl 2-amino-2-deoxy-β-D-glucopyranoside called C₁₈ was synthesized. The interaction between C₁₈and GOD (glucose oxidase) was studied by recording isotherms of monolayers. The transferred mixed C₁₈/GOD LB films were investigated by means of spectroscopy methods. From the results of CD (circular dichroism) spectrum,GOD immobilized in C₁₈films will partly change their secondary structure. According to the low temperature fluorescence experiment,part of GOD in the LB film keeps native structure,others of them become partly denatured.

Key words: glucose oxidase, circular dichroism spectrum, fluorescence spectrum, LangmuirBlodgett(LB) film

DU Yukou, TANG Ji’an, JIANG Long, Paul Boullanger. A SPECTROSCOPY STUDY OF THE MIXED LANGMUIR BLODGETT FILMS OF ALKYL GLYCOSIDE WITHPOSITIVE CHARGE AND GLUCOSE OXIDASE[J]. Imaging Science and Photochemistry, 1998, 16(1): 32-37.

References

1 Baker S A,Shinley J A.Economic Microbiology.Vol.5.New York:Academic Press,1980.445~466
2 Tsuge H,Natsuaki O,Ohashi K.Purification,properties and molecular features of glucose ox idase from as-pergillus niger.J.Biochem.,1975,78(4):835~843
3 Frederick K R,Tung J,Masiarz R S,et. al.Glucose oxidase from aspergillus niger.J.B iol.Chem.,1990,265:3793~3808
4 Weibel M K,Bright H J.The glucose oxidase mechanism.J.Biochem.,1971 246(9):2734~2744
5 Fisher J,Spencer R,Walsh C.Enzyme-catalyzed redox reactions with the flavin analogues 5-deazariboflavin,5-deazariboflavin 5′-phosphat e,and 5-deazariboflavin 5′-diphosphate,5′-adenosine ester.Biochemistry,1976,15(5):1054~1064
6 Fiol C,Valleton J M,Delpire N,et al.Thin Solid Film,1992,210:489~492
7 Li J R,Cai M,Chen T F,et al.Enzyme electrodes with conductive polymer membranes and Langmuir-Blodgett films.Thin Solid Film,1989,180:205~210
8 Okahata Y,Tsukuta T,Ijiro K,et al.Langmuir-Blodgett films of an enzyme-lipid complex for sensor mem-branes.Langmuir,1988,4:1373~1375
9 Okahata Y,Tsukuta T,Ijiro K,et al.Preparations of Langmuir-Blodgett films of enzyme-lipid complex s:A glu-cose sensor membrane.Thin Solid Films,1989,180:65~72
10 Sriyudthsak M,Yamagishi H,M oriizum T.Enzyme-immobilized Langmuir-Blodgett film for a biosensor.Thin Solid Films,1988,160:463~469
11 Ancelin H,Zhu D G,Petty M C,et al.Infrared spectroscopic studies of molecular structure ordering and inter-actions in enzyme-containing Langmuir-Blodgett films.Langmuir,1990,1068~1070
12 Boullanger P,Chevalier Y.Surface active properties and micellar aggregation of alkyl 2-amino-2-deox y--D-glu-copyranosides.Langmuir,1996,12:1771~1776
13 Fiol C,Alexandre S,Dubreuil N,et al.Characterization of behenic acid/glucose oxidase Langmuir-Blodgett films by spectrophotometry:a tentative model of their organization.Thin Solid Films,1995,261:287~295
14 Liu M H,Nakahara H,Shibasaki Y,et al.Conformational behaviors of synthetic copoly peptides in s preadmonolayers,built-up multilayers,and adsorbed films.J.Colloid Interface Sci.,1994,165:491~496
15 Hechet H J,Kalisz H M,Hendle J,et al.Crystal structure of glucose oxidase from A spergillus niger refined at 2.3A resolution.J.Mol.Biol.,1993,229:153~172
16 Swoboda B E P.The relationship between molecular conformation and binding of flavine-adenine dinucleotide inglucose oxidas e.Biochim.Biophys.Acta,1969,165:365~379
17 Tsuge H,Mitsuda H.The properties of glucose oxidase.J.V itaminol,1971,17:24~37
18 Ghisla S,Massey V,Lhoste J M,et al.Glucose oxidase from aspergillus niger.Biochemistry,1974,13:589~563
19 Frederick K R,Tung J,Emerick R S,et al.Glucose oxidase from aspergillus niger,cloning,gene sequence,se-cretion from saccharomyces cerevisiae and kinetic analys is of a yeast-drived enzyme.J.B iol.Chem.,1990,265:3793~3802
20 Trettnak W,Wolfbeis O.Fully reversiblefibre-optic glucose biosensor based on the intrinsic fluores cence of glu-cose ox idase.A nalytica Chimica A cta,1989,211:195~203
21 Lakowicz J R.Principles of Fluorescence Spectroscop y.New York:Plenum Press,1983.254~258

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