[an error occurred while processing this directive]
影像科学与光化学
 
Home | About Journal | Editorial Board | Instruction | Subscription | Awards and Included | advertisement | Messages | Contact Us | 中文
  Office Online
Editor Work
Peer Review
Office Work
Editor-in-chief
  Author Center
Online Submission
Manuscript Tracking
Scopes
Reference Format
Manuscript Modify
  Journal Online
Forthcoming Articles
Current Issue
Next Issue
Archive
Advanced Search
Read Articles
Download Articles
Email Alert
RSS
 
Quick Search  
  Advanced Search
Imaging Science and Photochemistry
 
2018 Vol.36 Issue.6
Published 2018-11-15

0
2018 Vol. 36 (6): 0-0 [Abstract] ( 16 ) [HTML 1KB] [ PDF 239KB] ( 36 )
467 Preperation of Gene-loaded Quantum Dots as Self-illuminating Probes for Cell Imaging
YUAN Chenyan, AN Yanli
To identify whether the CS-Qdots could act as gene carriers and self-illuminating probes simultaneously as delivering luciferase genes to cells. CdTe quantum dots were prepared and coated with chitosan to construct CS-Qdots nanoparticles. Then the CdTe and CS-Qdots nanoparticles were characterized; the excitation of CS-Qdots tested by bioluminescence in vivo. The reporter genes pCMV-luciferase were deliveried into cells by CS-Qdots and the wavelength of bioluminescence was tested to identify the pCMV-luciferase gene loaded CS-Qdots could act as the gene nanocarriers and self-illuminating probes simultaneously. The morphology of CdTe quantum dots and CS-Qdots nanoparticle were observed by TEM and were found to be spherical and good dispersion. The size of the fluorescent crystal was about 5 nm and the CS-Qdots nanoparticles were about 25-30 nm. When CdTe quantum dots were encapsulated by chitosan, the characteristic peaks of chitosan were observed on the FTIR spectra. The surface charge of CdTe-Qdots was -16.31±0.91 mV and then turned to a positive value of 28.02±1.15 mV. Dynamic light scattering measurements showed that the mean hydrodynamic diameter of CdTe quantum dots was 102.7±4.4 nm, and the mean hydrodynamic diameter of CS-Qdots nanoparticles was 583.0±13.7 nm. Spectral analysis showed that the CdTe quantum dots have wide absorption spectrum and the emission spectrum was narrow and symmetrical. By agarose gel electrophoresis, the plasmid pCMV-luciferase binding affinity of CS-Qdots was tested and the complete retardation was observed over the particle/DNA weight ratio of 10:1. In vivo test CS-Qdots could be directly excited by bioluminescent cells surrounded. When cells were transfected with pCMV-luciferase DNA by using CS-Qdots as gene carriers in vivo or in vitro, the emission peak of bioluminescence could red-shift from 560 to 630 nm, which means the CS-dots could be activated by bioluminescence. It is concluded that the luciferase gene-loaded CS-Qdots could act as wavelength-tunable self-illuminating probes and improve tumor optical molecular imaging.
2018 Vol. 36 (6): 467-477 [Abstract] ( 25 ) [HTML 1KB] [ PDF 6715KB] ( 88 )
478 Design, Synthesis and Properties of Highly Selective Fluorescent Probe for Methylamphetamine Vapor Detection
WANG Kaixia, XU Wei, HE Qingguo, CHENG Jiangong
Ice is one of the mainstream drugs in this century, effective detection and monitoring of ice is an important aspect of ensuring human health and maintaining social stability. The active ingredient of ice is methamphetamine (MA). Aiming at the structural characteristics of both benzene ring and the secondary amine in the MA molecule, two simple small molecule fluorescent probes, 5F-O-TPA and 5F-O-Py were designed and synthesized. The electrostatic attraction of pentafluorophenyl with phenyl ring, combined with the interaction between electron-withdrawing carbonyl groups and secondary electron-rich amines, two factors synergistically contribute to their strong intermolecular interaction, and hence a much efficient PET process and fluorescence quenching. It was found that the two probe molecules have a great difference in the sensing performance of MA. Because 5F-O-Py film has a large specific surface area and good permeability, it can be in good contact with MA molecules. Therefore, in the saturated MA vapor, the fluorescence quenched by 68% within 300 s, and the detection limit of MA vapor can theoretically reach 3.73 ppm, and still has a 38% quenching response after being reversibly used for 6 times. The sensory film has strong anti-interference ability, even its mimetics that are very similar to the MA structure can be effectively distinguished by 5F-O-Py.
2018 Vol. 36 (6): 478-488 [Abstract] ( 24 ) [HTML 1KB] [ PDF 5378KB] ( 61 )
489 Preparation and Properties of UV-cured Self-healing Polyurethane Coatings Based on Diels-Alder Reaction
CAO Jiancheng, LU Fukang, LIU Jingcheng, LIU Ren, YUAN Yan
N,N'-4,4'-Diphenylmethane-bismaleimide (BMI) and furfuryl alcohol (FA) were used to synthesize the modified DA monomers with hydroxyl groups through Diels-Alder reaction, and then use the polycarbonates diols (PCDL)with different molecular weights, isophorone isocyanate (IPDI) and modified DA monomers as reaction monomers to obtain a polyurethane prepolymer. Then the UV cured self-healing polyurethane were prepared by reaction of the prepolymer and hydroxyethyl methacrylate (HEMA). Fourier transform infrared (FT-IR) and nuclear magnetic resonance (1HNMR) spectra were used to characterize the structure and double bond conversion of the self-healing polyurethane. In addition, the UV cured self-healing polyurethane coatings were prepared by designing a series of coating formulations with the polyurethane. The self-healing properties and thermal properties of the coatings were tested by ultra-deep-depth microscope and thermal gravimetric analyzer (TGA). The results showed that the hardness of the coatings decreased as the molecular weight of PCDL increased, and the introduction of the DA monomer imparts self-healing properties to the coating while increasing the hardness of the coating.
2018 Vol. 36 (6): 489-497 [Abstract] ( 35 ) [HTML 1KB] [ PDF 3474KB] ( 41 )
498 Synthesis of Rare Earth Fluorescent Complexes Containing Europium and Their Applications in Latent Fingerprint Development
ZHANG Zhansheng, GAO Yangchen, SHEN Dunpu, XIE Yinpeng, LI Ming, WANG Meng
A series of rare earth fluorescent complexes containing Europium (Eu) were synthesized via a precipitation approach, using o-, m-, p-methylbenzoic acid (o-, m-, p-MA) as the first ligand, respectively, and o-phenanthroline (o-Phen) as the second ligand. Their synthetic conditions were also optimized systematically, including the category of ligands, the molar ratio of ligands, pH, reaction temperature, and reaction time. The powders of these as-prepared[Eu(m-MA)3(o-Phen)] complexes were then used to develop the latent fingerprints on various of smooth substrates. In addition, the contrast, sensitivity, and selectivity for fingerprint development were investigated in detail. The results showed that the contrast between the developed fingerprint and the substrate was quite high, under the excitation of 254 nm ultraviolet light. The friction ridges were coherent and sharp enough, even the minutiae features of the friction ridges could be clearly observed. The fluorescent developing powders were adsorbed only on the friction ridges, rather than the furrows. Therefore, our rare earth fluorescent complex based fingerprint development could exhibit strong contrast, high sensitivity, and good selectivity.
2018 Vol. 36 (6): 498-506 [Abstract] ( 19 ) [HTML 1KB] [ PDF 5783KB] ( 54 )
507 Research on Four Primary Color LED Hybrid White Light with Brightness Continuous Adjustable under Different Color Temperature
XU Xiangyang, CHEN Guangxue, GAO Yuan
In order to provide the lighting environment for quantifying the performance of camera-equipped mobile phone, the LED light source with adjustable color temperature and brightness have been developed in this paper. Red LED light source, green LED light source, cool white LED light source and warm white LED light source were selected as the four primary colors, and an experimental device was designed to realize the development of white light source with continuous adjustable color temperature and brightness. The color matching technology studied in this paper took the relative spectral power distribution, chromaticity coordinate, and luminous flux of the four primary as the input parameters. According to Grassmann's law, the color matching algorithm has been developed, the chromaticity coordinate of the Planckian locus are the function values, and the corresponding light flux are variables. In the model solution, the chromatic aberration △UV is the first optimization parameter, which should be less than 0.005. The first optimization has not been completed until the color difference is within the limit. Then the color rendering index(CRI) Ra is more than 90 as optimization target, and the calculation has not been carried out until the relative color temperature error is within 100. The test results of the developed light source showed that the color difference △UV is less than 0.003 and Ra is more than 91, when achieving the target color temperature and target brightness.
2018 Vol. 36 (6): 507-513 [Abstract] ( 20 ) [HTML 1KB] [ PDF 1660KB] ( 38 )
514 Synthesis and Spectrometric Detection of Lindqvist Acid and Aniline Hybrid Solid
ZHANG Yucai, CHEN Hongman, DENG Renpan, YAN Yin, MIAO Zhiwei
Using Lindqvist hexamolybdate as an inorganic composite and aniline as an organic composite, a covalently linked hexamolybdopolyacid-aniline hybrid solid was synthesized by dehydration method with dicyclohexyl carbodiimide. The compound was characterized by 1HNMR,13CNMR, IR and UV-Vis spectroscopy. The UV-Vis spectra of the compound showed that the apparent red-shifted band (about 37 nm) was observed compared with aniline, which confirmed that the triple bond formed between Mo and N, and further increased the conjugation of the system. IR spectra displayed that the compound kept the basic structure of Lindqvist polyacid. UV-Vis spectroscopy was used to detect different concentrations of Vitamin C. It was found that the absorbance of the hybrid solution didn't change significantly after repeated cycles, indicating that the UV-visible probe can be recycled.
2018 Vol. 36 (6): 514-521 [Abstract] ( 27 ) [HTML 1KB] [ PDF 769KB] ( 72 )
522 Preparation and Application of O-cresol Formaldehyde Epoxy Acrylates Resin Modified by Cardanol Glycidyl Ether
XU Yangyang, LI Zhiquan, AN Fenglei, YUAN Yanhua, LIU Ren
An o-cresol formaldehyde epoxy acrylates resin modified by cardanol glycidyl ether (EATC) was prepared and used in solder resist ink. The synthesis of EATC was studied and characterized in detail by IR spectroscopy and 1HNMR spectrum.The coating made of modified resin exhibited good photosensitive, thermal and mechanical properties. Besides, the flexibility of the film became better. Compared to unmodified o-cresol formaldehyde epoxy acrylates resin, the solder resist ink which consist of EATC has good performance in photosensitivity. The resolution increased from 140 to 85 μm,and the minimal etching resistance decrease from 37.1 to 10.4 μm.
2018 Vol. 36 (6): 522-531 [Abstract] ( 22 ) [HTML 1KB] [ PDF 6133KB] ( 36 )
532 Common Problems of Confocal Laser Scanning Microscopy on Colocalization
WANG Juanjuan, WEI Xuehong
The colocalization of fluorescence is a common technique in the biological imaging. In order to get true results of colocalization, we describe the following four aspects in detail, which are common problems of colocalization by Zeiss LSM880 (confocal laser scanning microscope, CLSM), as well as the solutions. First, how to use CLSM for colocalization experiments. Second, how to conduct colocalization analysis. Third, how to evaluate colocalization. Fourth, how to determine the colocalization results. In the colocalization study, we can get the accurate and reliable colocalization analysis results if we take the images strictly following the basic requirements of the colocalization experiment and analyse them according to the above methods.
2018 Vol. 36 (6): 532-538 [Abstract] ( 21 ) [HTML 1KB] [ PDF 3413KB] ( 38 )
539 Combining Variance Stable Transform and PGPD to Remove Rician Noise from Magnetic Resonance Images
ZHANG Ruonan, CHI Yue, ZHANG Wei, CUI Tao
The patch group based non-local self-similarity prior learning for image denosing (PGPD) algorithm has excellent effect of removing Gaussian noise, but the removal effect of Rician noise in MR images is not ideal. In this paper, a new algorithm FPGPD combining variance stable transform and PGPD is proposed. Firstly, the algorithm performs the variance-stable transformation on the MR image with Rician noise, so that the noise approximation obeys the Gaussian distribution in the transform domain. Then, using the PGPD algorithm to denoise in the transform domain, and finally obtains the unbiased denoising image through the inverse variance transform. Theoretical analysis and experimental results indicate that the FPGPD algorithm has significantly improved denoising performance compared to the PGPD algorithm when removing Rician noise in MR images, which is better for image detail and contour edges.
2018 Vol. 36 (6): 539-550 [Abstract] ( 21 ) [HTML 1KB] [ PDF 6448KB] ( 35 )
影像科学与光化学
  News
More...
  Links
 
 
 
 
 
 
More... 
  Download
   
   
   
   Download
More...  
Copyright © 2012 Editorial by Imaging Science and Photochemistry
Address: 29 Zhongguancun East Road,Beijing 100190,P.R.China Tel:86-10-82543683 E-mail:xb@mail.ipc.ac.cn
Support by  Beijing Magtech