Multimodal Imaging Based on Transport of Intensity Equation

doi:10.7517/j.issn.1674-0475.2017.02.179

Imaging Science and Photochemistry ›› 2017, Vol. 35 ›› Issue (2): 179-184.DOI: 10.7517/j.issn.1674-0475.2017.02.179

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Multimodal Imaging Based on Transport of Intensity Equation

ZHANG Zhao^1,2, LI Jiaji^1,2, SUN Jiasong^1,2, ZHANG Jialin^1,2, ZUO Chao^1,2

1. Smart Computational Imaging Laboratory, School of Electronics and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu, P. R. China;
2. Jiangsu Province Key Laboratory of Spectral Imaging and Intelligence Sense, School of Electronics and Optical Engineering, Nanjing University of Science and Technology, Nanjing 210094, Jiangsu, P. R. China

Received:2017-01-20 Revised:2017-02-15 Online:2017-03-15 Published:2017-03-15

Abstract

Abstract:

Transport of intensity equation (TIE) is a powerful tool for phase retrieval and quantitative phase imaging,which requires a series of intensity images along the optical axis. The numerical difference is used to estimate the first order axial differential of light intensity at the focal plane, and the phase information can be obtained directly by solving the transport of intensity equation. It does not require coherent illumination and works well on conventional bright-field microscopes. In recent years, Transport of intensity equation has been widely studied at home and abroad. It shows great application prospect in the fields of adaptive optics, X-ray diffraction optics, electron microscopy and optical microscopic imaging. This paper proposes a multi-mode imaging system based on transport of intensity equation, which combines the traditional microscope and the computational imaging, and gives the ability of obtaining the quantitative phase to the traditional microscope.The versatility and effectiveness of the system are demonstrated by microexamination of several transparent colorless specimens, such as unstained pollen grains and live HeLa cells.

Key words: phase retrieval, transport of intensity equation, quantitative phase, multimodal imaging

ZHANG Zhao, LI Jiaji, SUN Jiasong, ZHANG Jialin, ZUO Chao. Multimodal Imaging Based on Transport of Intensity Equation[J]. Imaging Science and Photochemistry, 2017, 35(2): 179-184.

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Multimodal Imaging Based on Transport of Intensity Equation

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