Study on Fluorescence Characteristics of Chitosan-supported Cyanine Dye Fluorescence Probes

doi:10.7517/j.issn.1674-0475.2009.04.307

Imaging Science and Photochemistry ›› 2009, Vol. 27 ›› Issue (4): 307-313.DOI: 10.7517/j.issn.1674-0475.2009.04.307

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Study on Fluorescence Characteristics of Chitosan-supported Cyanine Dye Fluorescence Probes

LIU Zhi-jun, FEI Xue-ning, GU Ying-chun

Department of Material Science and Engineering, Tianjin Institute of Urban Construction, Tianjin 300384, P. R. China

Received:2008-12-01 Revised:2009-01-07 Online:2009-07-23 Published:2009-07-23

Abstract

Abstract: Organic fluorescent Dyes can be supported by Chitosan and the fluorescent Characteristics in physiological environment can be changed.In this paper,unsymmetrical water-soluble cyanine dye(Cy3) was supported by Chitosan(CTS),and the product(Cy3-CTS) was characterized by IR and DTA.The fluorescence Characteristics and ultraviolet absorbance were studied.The results showed that the characteristic peak of UV absorption spectrum of Cy3-CTS shifted little compared to that of Cy3,but the fluorescence intensity enhanced obviously with good photostability.Primary experimental study on labeled cells indicated that Cy3-CTS can pass through the cell membrane with good affinity to glioma cell and show strong fluorescence.

Key words: Cy3, chitosan-supported, fluorescence characteristic, ultraviolet absorption, labeled cells

CLC Number:

TQ57

LIU Zhi-jun, FEI Xue-ning, GU Ying-chun. Study on Fluorescence Characteristics of Chitosan-supported Cyanine Dye Fluorescence Probes[J]. Imaging Science and Photochemistry, 2009, 27(4): 307-313.

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Study on Fluorescence Characteristics of Chitosan-supported Cyanine Dye Fluorescence Probes

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