影像科学与光化学 ›› 1995, Vol. 13 ›› Issue (1): 53-60.DOI: 10.7517/j.issn.1674-0475.1995.01.53

• 研究论文 • 上一篇    下一篇

红藻条斑紫菜R-藻红蛋白中的能量传递过程

伍华菊1, 张建平1, 夏安东1,2, 朱晋昌1, 蒋丽金1   

  1. 1. 中国科学院感光化学研究所, 北京100101;
    2. 合肥中国科学技术大学国家同步辐射实验室
  • 收稿日期:1994-03-23 修回日期:1994-07-13 出版日期:1995-02-20 发布日期:1995-02-20
  • 通讯作者: 蒋丽金
  • 基金资助:
    “八五”重大基础研究项目;国家自然科学基金

ENERGY TRANSFER IN R-PHYCOERYTHRIN FROM RED ALGA PROPHYRA YEZOENSIS

WU HUA-JU1, ZHANG JIAN-PING1, XIA AN-DONG1,2, ZHU JING-CHANG1, JIANG LI-JIN1   

  1. 1. Institute of Photographic Chemistry, The chinese Academy of Sciences, Beijing 100101, P.R. China
  • Received:1994-03-23 Revised:1994-07-13 Online:1995-02-20 Published:1995-02-20

摘要: 本文通过对条斑紫菜R-PE(藻红蛋白)及其α-β-γ亚基的吸收光谱和荧光光谱进行计算机解叠,研究了R-PE内发色团之间的能量传递过程,并对R-PE及亚基内的各发色团进行了“s”和“f”型的指认。发现在亚基中为“f”型的发色团在R-pE(αβ)6γ中起着“s”型发色团的作用,且将能量传递给最后的“f”型发色团。荧光激发偏振光谱进一步证明了R-PE内的能量转移过程与计算机解叠的结果一致。

关键词: 光谱解叠, 能量传递, 藻胆蛋白

Abstract: The energy transfer processes ih R-phycoerythrin as well as its α,β,γ,subunits isolated from red alga prophyra yezoensis were studied by absorption, fluorescence spectra and the deconvolution of these spectra.A tentative assignment of“s”and“f”type chromophores in R-PE and α,β,γ subunits was also maken, The“f”type chromophore in β(or γ)subunitis found to be an s-type chromophore in R-PE[(αβ)6γ], and transfer its energy to the final“f”type chromophores. The results obtained from the fluorescence excitation polarization spectrum of R-PE are in good agreement with the deconvolution results for the energy transfer processes.

Key words: deconvolution, energy transfer, phycobiliprotein