藻蓝蛋白对Au(Ⅲ)原位还原与纳米Au(0)形成动态过程的谱学研究

doi:10.7517/j.issn.1674-0475.2006.02.118

影像科学与光化学 ›› 2006, Vol. 24 ›› Issue (2): 118-125.DOI: 10.7517/j.issn.1674-0475.2006.02.118

藻蓝蛋白对Au(Ⅲ)原位还原与纳米Au(0)形成动态过程的谱学研究

杨芳^1,2, 郭振江¹, 白燕¹, 黄峙³, 郑文杰^1,2

1. 暨南大学, 化学系, 广东, 广州, 510632;
2. 暨南大学, 水生生物研究所, 广东, 广州, 510632;
3. 暨南大学, 生物工程学系, 广东, 广州, 510632

收稿日期:2005-10-14 修回日期:2005-12-09 出版日期:2006-03-23 发布日期:2006-03-23
通讯作者: 郑文杰,tzhwj@jnu.edu.cn.
基金资助:
广东省自然科学基金重点项目(05103295);广东省自然科学基金团队项目(039213)

Spectra of in Situ Reduction of Au(Ⅲ) by Phycocyanin and Dynamic Formation of Au(0)

YANG Fang^1,2, GUO Zhen-jiang¹, BAI Yan¹, HUANG Zhi³, ZHENG Wen-jie^1,2

1. Department of Chemistry, Jinan University, Guangzhou 510632, Guangdong, P. R. China;
2. Institute of Hydrobiology, Jinan University, Guangzhou 510632, Guangdong, P. R. China;
3. Department of Biotechnology, Jinan University, Guangzhou 510632, Guangdong, P. R. China

Received:2005-10-14 Revised:2005-12-09 Online:2006-03-23 Published:2006-03-23

摘要/Abstract

摘要： 在4℃、避光条件下,将藻蓝蛋白(PC)与AuCl₃作用,通过UV、FS、FT-IR等谱学方法研究了PC与Au(Ⅲ)作用的动态过程,结果显示:PC在615nm的特征吸收峰强度明显减弱,并随Au(Ⅲ)浓度增加和时间延长单调降低;PC的荧光发射峰和荧光激发峰也均呈现衰减趋势.同时通过TEM观察到纳米Au(0)粒子形成,纳米金基本呈球形,有较窄的分布尺寸,粒径在20 nm左右,均匀地分散于PC中,这是PC原位还原Au(Ⅲ)所形成的.

关键词: 钝顶螺旋藻, 藻蓝蛋白, 光谱, 纳米金

Abstract: The interaction of Au(Ⅲ) with Phycocyanin(PC) was investigated by ultraviolet-visible,fluorescence and infrared spectra under the condition of 4 ℃ and without light.The results showed that the characteristic absorption of PC at 615 nm decreased gradually with the increase of Au(Ⅲ) concentration and interaction time.Accordingly,its fluorescence emission peak and two fluorescence excitation peaks also decreased.It was also observed by TEM that nano-Au(0) was formed by the PC in situ reduction.The diameter of Au(0) was about 20 nm,which was round,highly dispersed and stable in PC.

Key words: spirulina platensis, phycocyanin, spectroscopy, nano-aurum

中图分类号:

杨芳, 郭振江, 白燕, 黄峙, 郑文杰. 藻蓝蛋白对Au(Ⅲ)原位还原与纳米Au(0)形成动态过程的谱学研究[J]. 影像科学与光化学, 2006, 24(2): 118-125.

YANG Fang, GUO Zhen-jiang, BAI Yan, HUANG Zhi, ZHENG Wen-jie. Spectra of in Situ Reduction of Au(Ⅲ) by Phycocyanin and Dynamic Formation of Au(0)[J]. Imaging Science and Photochemistry, 2006, 24(2): 118-125.

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藻蓝蛋白对Au(Ⅲ)原位还原与纳米Au(0)形成动态过程的谱学研究

Spectra of in Situ Reduction of Au(Ⅲ) by Phycocyanin and Dynamic Formation of Au(0)

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