Application of Spectral CT Combined with Long Non-coding RNA UCA1 in the Diagnosis of Lymph Node Metastasis of Gastric Adenocarcinoma

doi:10.7517/issn.1674-0475.220722

Abstract

Abstract: This study investigated the value of spectral CT parameters combined with long non-coding RNA (lncRNA) urothelial carcinoma-associated gene 1 (UCA1) in the diagnosis of lymph node metastasis in gastric adenocarcinoma. A total of 103 patients with gastric adenocarcinoma were selected as the research objects. All patients underwent spectral CT examination to obtain the normalized iodine concentration value in the arterial phase (NICAP) and the normalized iodine concentration value in the venous phase (NICVP). Serum UCA1 levels in patients with gastric adenocarcinoma were detected by RT-PCR. The results showed that NICAP and NICVP in the metastatic group were significantly higher than those in the non-metastatic group (P＜0.001). Taking NICAP>0.132 and NICVP>0.431 as cutoff values, the AUC, sensitivity and specificity of NICAP and NICVP for the diagnosis of lymph node metastasis were (0.742, 68.52% and 76.09%) and (0.932, 85.19% and 86.96%), respectively. The relative expression of serum UCA1 in the metastatic group was significantly higher than that in the non-metastatic group (P＜0.001). Taking the relative expression of serum UCA1>2.071 as the cut-off value, the AUC, sensitivity and specificity of serum UCA1 in the diagnosis of lymph node metastasis were 0.884, 87.04% and 82.61%, respectively. The AUC, sensitivity and specificity of NICAP+NICVP+UCA1 in the diagnosis of lymph node metastasis were 0.985, 94.44% and 100.00%, respectively. Spectral CT parameters (NICAP and NICVP) combined with serum UCA1 have high value in the diagnosis of lymph node metastasis of gastric adenocarcinoma.

Key words: spectral CT, gastric adenocarcinoma, lymph node metastasis, NICAP, NICVP, UCA1

ZHANG Jian, REN Xiaoyan. Application of Spectral CT Combined with Long Non-coding RNA UCA1 in the Diagnosis of Lymph Node Metastasis of Gastric Adenocarcinoma[J]. IMAGING SCIENCE AND PHOTOCHEMISTRY, 2022, 40(6): 1539-1544.

References

[1] Dong D, Tang L, Li Z Y, et al. Development and validation of an individualized nomogram to identify occult peritoneal metastasis in patients with advanced gastric cancer[J]. Annals of Oncology, 2019, 30(3):431-438.
[2] Wang Y, Liu W, Yu Y, et al. CT radiomics nomogram for the preoperative prediction of lymph node metastasis in gastric cancer[J]. European Radiology, 2020, 30(2):976-986.
[3] Li J, Dong D, Fang M J, et al. Dual-energy CT-based deep learning radiomics can improve lymph node metastasis risk prediction for gastric cancer[J]. European Radiology, 2020, 30(4):2324-2333.
[4] Zhang W J, Fang M J, Dong D, et al. Development and validation of a CT-based radiomic nomogram for preoperative prediction of early recurrence in advanced gastric cancer[J]. Radiotherapy and Oncology, 2020, 145:13-20.
[5] Saito T, Kurokawa Y, Takiguchi S, et al. Accuracy of multidetector-row CT in diagnosing lymph node metastasis in patients with gastric cancer[J]. European Radiology, 2015, 25(2):368-374.
[6] Clark D P, Lee C L, Kirsch D G, et al. Spectrotemporal CT data acquisition and reconstruction at low dose[J]. Medical Physics, 2015, 42(11):6317-6336.
[7] 李祖银, 朱迪, 夏春华. 能谱CT扫描CT值、IC值在胃腺癌淋巴结定性评估中的临床应用[J]. 中国医药导报, 2021, 18(4):147-150, 183.
[8] 王睿, 李靖, 梁盼, 等. 能谱CT术前预测胃腺癌淋巴结转移的价值[J]. 临床放射学杂志, 2019, 38(3):469-474.
[9] 周岩, 王云, 杨晓晔, 等. 能谱CT用于胃癌术前肿瘤浸润深度和淋巴结转移的诊断价值研究[J]. 现代消化及介入诊疗, 2019, 24(12):1481-1484.
[10] Lin M T, Song H J, Ding X Y. Long non-coding RNAs involved in metastasis of gastric cancer[J]. World Journal of Gastroenterology, 2018, 24(33):3724-3737.
[11] Wei F, Wang Y, Zhou Y, et al. Long noncoding RNA CYTOR triggers gastric cancer progression by targeting miR-103/RAB10[J]. Acta Biochimica et Biophysica Sinica, 2021, 53(8):1044-1054.
[12] Zhang H, Ma R R, Zhang G H, et al. Long noncoding RNA lnc-LEMGC combines with DNA-PKcs to suppress gastric cancer metastasis[J]. Cancer Letters, 2022, 524:82-90.
[13] Yao F, Wang Q, Wu Q M. The prognostic value and mechanisms of lncRNA UCA1 in human cancer[J]. Cancer Management and Research, 2019, 11:7685-7696.
[14] Yang A, Liu X, Liu P, et al. LncRNA UCA1 promotes development of gastric cancer via the miR-145/MYO6 axis[J]. Cellular & Molecular Biology Letters, 2021, 26(1):33.
[15] Dai Q Q, Zhang T Q, Pan J M, et al. LncRNA UCA1 promotes cisplatin resistance in gastric cancer via recruiting EZH2 and activating PI3K/AKT pathway[J]. Journal of Cancer, 2020, 11(13):3882-3892.
[16] Dicken B J, Bigam D L, Cass C, et al. Gastric adenocarcinoma:Review and considerations for future directions[J]. Annals of Surgery, 2005, 241(1):27-39.
[17] Yan S Y, Hu Y, Fan J G, et al. Clinicopathologic significance of HER-2/neu protein expression and gene amplification in gastric carcinoma[J]. World Journal of Gastroenterology, 2011, 17(11):1501-1506.
[18] Bang Y J, Kang Y K, Kang W K, et al. Phase II study of sunitinib as second-line treatment for advanced gastric cancer[J]. Investigational New Drugs, 2011, 29(6):1449-1458.
[19] 马轶, 梁青, 武婧, 等. 64排螺旋CT胃癌征象与病理分期及组织分化程度的相关性[J]. 医学影像学杂志, 2016, 26(5):824-828.
[20] Liu S L, Liu S, Ji C F, et al. Application of CT texture analysis in predicting histopathological characteristics of gastric cancers[J]. European Radiology, 2017, 27(12):4951-4959.
[21] Shen Y L, Kang H K, Jeong Y Y, et al. Evaluation of early gastric cancer at multidetector CT with multiplanar reformation and virtual endoscopy[J]. Radiographics:a Review Publication of the Radiological Society of North America, Inc, 2011, 31(1):189-199.
[22] Meng X Y, Ni C, Shen Y Q, et al. Differentiating malignant from benign gastric mucosal lesions with quantitative analysis in dual energy spectral computed tomography:Initial experience[J]. Medicine, 2017, 96(2):e5878.
[23] Pan Z L, Pang L F, Ding B, et al. Gastric cancer staging with dual energy spectral CT imaging[J]. PLoS One, 2013, 8(2):e53651.
[24] Chen X H, Ren K, Liang P, et al. Spectral computed tomography in advanced gastric cancer:Can iodine concentration non-invasively assess angiogenesis?[J]. World Journal of Gastroenterology, 2017, 23(9):1666-1675.
[25] Ba M C, Ba Z, Long H, et al. LncRNA AC093818.1 accelerates gastric cancer metastasis by epigenetically promoting PDK1 expression[J]. Cell Death & Disease, 2020, 11(1):64.
[26] Schmitt A M, Chang H Y. Long noncoding RNAs in cancer pathways[J]. Cancer Cell, 2016, 29(4):452-463.
[27] Niu J T, Song X X, Zhang X M. Regulation of lncRNA PVT1 on miR-125 in metastasis of gastric cancer cells[J]. Oncology Letters, 2020, 19(2):1261-1266.
[28] Liu C M, Jin J, Shi J, et al. Long noncoding RNA UCA1 as a novel biomarker of lymph node metastasis and prognosis in human cancer:A meta-analysis[J]. Bioscience Reports, 2019, 39(4):BSR20180995.
[29] He A B, Hu R, Chen Z C, et al. Role of long noncoding RNA UCA1 as a common molecular marker for lymph node metastasis and prognosis in various cancers:A meta-analysis[J]. Oncotarget, 2017, 8(1):1937-1943.
[30] Wang Y H, Wang F, Zhang L, et al. Long non-coding RNA UCA1 can predict tumor lymph node metastasis[J]. Tumour Biology, 2017, 39(5):1010428317706208.
[31] Gong P H, Qiao F C, Wu H Z, et al. LncRNA UCA1 promotes tumor metastasis by inducing miR-203/ZEB2 axis in gastric cancer[J]. Cell Death & Disease, 2018, 9(12):1158.