STUDY ON CORRELATION BETWEEN ·OH,O2·- RADICALSAND LOW-LEVEL CHEMILUMINESCENCE FROMMITOCHONDRIA

doi:10.7517/j.issn.1674-0475.1994.04.312

Imaging Science and Photochemistry ›› 1994, Vol. 12 ›› Issue (4): 312-317.DOI: 10.7517/j.issn.1674-0475.1994.04.312

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STUDY ON CORRELATION BETWEEN ^·OH,O₂^·- RADICALSAND LOW-LEVEL CHEMILUMINESCENCE FROMMITOCHONDRIA

MA YU-QIN, ZHAO KE-JIAN, ZHANG ZHONG-LUN, SU ZHEN, MA BIN, ZHENG YAN-ZHEN, YANG GUANG-JIE, MI ZHI-YONG, CHEN XIN

Institute of Biophysics, Academia Sinica, Beijing 100101, P. R. China

Received:1994-01-12 Revised:1994-04-21 Online:1994-11-20 Published:1994-11-20

Abstract

Abstract: Mitochondria was used a sa model system to study the course and mechanism of low-level chemiluminescence.It is discovered that the LCL of mitochondria suspension riseswith its concentration increasing under the definited range (0-100 mg/mL protein).Thebest satisfactory condition is 35℃ and PH=7.4.By adding of exogenous ^·OH and ^·OH cleaner, it is convicted that ^·OH can initiate lipld peroxidation reactions and ¹O₂ is one of the main photon sources.SOD(in vivo) may adjust the LCL from mitochondria.

Key words: mitochondria, low-level chemiluminescence, activated oxygen

MA YU-QIN, ZHAO KE-JIAN, ZHANG ZHONG-LUN, SU ZHEN, MA BIN, ZHENG YAN-ZHEN, YANG GUANG-JIE, MI ZHI-YONG, CHEN XIN. STUDY ON CORRELATION BETWEEN ^·OH,O₂^·- RADICALSAND LOW-LEVEL CHEMILUMINESCENCE FROMMITOCHONDRIA[J]. Imaging Science and Photochemistry, 1994, 12(4): 312-317.

STUDY ON CORRELATION BETWEEN ^·OH,O₂^·- RADICALSAND LOW-LEVEL CHEMILUMINESCENCE FROMMITOCHONDRIA

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