Detection of G-quadruplex by Supramolecular Systems Assembled by Perylenediimide Derivatives and Terpyridineplatinum(Ⅱ) Complexes

doi:10.7517/j.issn.1674-0475.2017.04.017

Abstract

Abstract:

Three terpyridine platinum complexes (Pt1-3) and two perylenediimide derivatives (PDI1-2) are synthesized, and employed to construct "turn-on" type fluorescent sensing ensembles for c-myc G-quadruplex. The fluorescence of perylenediimide derivatives can be efficiently quenched due to photoinduced electron transfer (PET) from platinum complex to perylenediimide. The ensembles exhibit moderate association constants in the range of 5.57×10⁴~5.28×10⁶ L/mol calculated by nonlinear least-squares curve-fitting method. Addition of G-quadruplex to the ensembles, the fluorescence of perylenediimide can be recovered due to the dissociation of the ensembles. For the Pt2/PDI2 system, the emission enhancement (F/F₀) is about 63-fold when the concentration of c-myc G-quadruplex is 1.5 μmol/L. The fluorescence enhancement shows an excellent linear relationship (R² = 0.995) with the concentration of c-myc in the range of 25 nmol/L~1.0 μmol/L, and the limit of detection is 1.37 nmol/L, indicating that the Pt2/PDI2 ensemble is sensitive to c-myc.

Key words: platinum (Ⅱ) complex, G-quadruplex, perylenediimides, fluorescence sensor

GAO Yunyan, CAI Wenjiao, OU Zhize, MA Tuotuo, WANG Ziji, XU Moheng. Detection of G-quadruplex by Supramolecular Systems Assembled by Perylenediimide Derivatives and Terpyridineplatinum(Ⅱ) Complexes[J]. Imaging Science and Photochemistry, 2017, 35(4): 552-562.

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